PhD Scientific Days 2024

Poster Session A - Molecular Medicine 1.

Studying Peroxidasin Function in Adipose Tissue Homeostasis

Előadó neve

Ms. Balog, Beáta

Neptun code

A0Z6ZZ

Előadó munkahelye

Semmelweis University, Dept. of Physiology

Előadó telefonszáma

+36204908077

Előadó e-mail címe

balog.beata.adel@semmelweis.hu

Az előadás címe

Studying Peroxidasin Function in Adipose Tissue Homeostasis

Szerző(k) neve és munkahelye

Beáta Balog¹, Gábor Sirokmány¹, Bernadett Balázs¹, Lili Hegedüs¹, Panna Keszte¹, Nóra Jassó¹

1: Semmelweis University, Dept. of Physiology

Bemutatás módja

Poszter

Szekció

Poster Session A - Molecular Medicine 1.

Language of the presentation

Hungarian

Preferred session

Molecular Medicine

Összefoglaló szövege

Introduction and aim:
Peroxidasin (PXDN) is a mammalian heme peroxidase that catalyses covalent cross-linking of the NC1 domains of the extracellular matrix protein collagen IV. Adipose tissue expresses Col4a1 and Col4a2 isoforms as well as PXDN in high amounts. In adipose tissue, each fat cell is surrounded by a collagen IV-rich extracellular matrix. Our aim was to study our PXDN deficient animal model to reveal the possible function of this peroxidase in the physiology of adipose tissue.

Methods:
We developed both PXDN knockout and mutant (inactive) PXDN knockin animal models. We used MRI scan to assess body fat percentages. We analyzed tissue morphology on paraffin embedded sections of perigenital adipose tissue. Trizol based RNA extraction was used to isolate RNA from adipose tissue and we checked gene expression of several WAT and BAT markers using standard Taqman qPCR assays. We also analyzed the changes of PXDN and collagen IV protein levels during adipogenic differentiation of 3T3-L1 cells.

Results:
Peroxidasin deficient mice display significantly reduced body fat percentage in both the visceral and subcutaneous compartment and a lower body weight compared to their wild-type littermates. Histological analysis revealed pronounced changes in tissue morphology in Pxdn-knockout animals. The average fat cell area was significantly reduced in PXDN-knockout animals. In vitro, 3T3-L1 cells express PXDN throughout the adipogenic differentiation process, however, collagen IV expression increases only after 2-3 days of incubation in insulin containing differentiation medium. Inhibition of peroxidase activity of PXDN by phloroglucinol did not inhibit adipogenic differentiation of 3T3-L1 cells.

Conclusion:
Peroxidasin might have a hitherto unrecognized role in the differentiation and maintenance of adipose tissues. Biochemical changes in extracellular matrix components around the adipocytes might play a significant role in PXDN-dependent effects. Further in vitro and in vivo studies are necessary to understand the exact molecular mechanisms of PXDN’s actions.

E-mail address: balog.beata.adel@semmelweis.hu
Name of the University: Semmelweis University, Dept. of Physiology
Name of the Supervisors: Gábor Sirokmány, Miklós Geiszt

University

Semmelweis University

Supervisor

Gábor Sirokmány, Miklós Geiszt

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

8103

Start

15:35

End

15:38