PhD Scientific Days 2024

Poster Session D - Neurosciences 1.

Screening AAV delivery routes, capsids and promoters for cortex-wide functional and long-term stable access to brain function in large-brain species

Előadó neve

Fanni, Somogyi, MSc

Neptun code

E58ZQ3

Előadó munkahelye

HUN-REN Research Centre for Natural Sciences

Előadó telefonszáma

06702686813

Előadó e-mail címe

vereslfanni@gmail.com

Az előadás címe

Screening AAV delivery routes, capsids and promoters for cortex-wide functional and long-term stable access to brain function in large-brain species

Szerző(k) neve és munkahelye

Fanni Somogyi^1,2, Beatrix Kovacs^1,2, Klaudia Csikos^1,2, Abel Petik^1,3, Domonkos Horvath^1,3, Attila B. Dobos¹, Lucia Wittner¹, Daniel Hillier^1,3

1: HUN-REN Research Centre for Natural Sciences
2: János Szentágothai Neurosciences Program, Semmelweis University Doctoral School
3: Faculty of Information Technology and Bionics, Pázmány Péter Catholic University

Bemutatás módja

Poszter

Szekció

Poster Session D - Neurosciences 1.

Language of the presentation

English

Preferred session

Neurosciences

Összefoglaló szövege

How signals relevant to behavior and cognition are processed within neural circuits, engaging thousands of neurons across multiple brain regions remains a central question. After decades of research, our current understanding of brain-wide neural circuits enabling various brain functions in non-rodent species (e.g., cats, primates) remains limited. In stark contrast, significant progress has been made in dissecting mouse brain function, enabled by the availability of transgenic driver and reporter lines. We set out to emulate the quality and reproducibility of transgenic mouse reporter lines in non-rodent models by establishing a long-term stable gene delivery method that achieves functional protein levels across the brain. We quantified transduction efficiency upon screening a set of constructs in the cat brain. We identified one construct that yields brain-wide labeling upon a single injection. Our result provides a precise, highly reproducible brain-wide transduction method, potentially approximating the qualities attributed to transgenic reporter mouse lines. Using this method, genetically-targeted dissection of both local and brain-wide functional circuits may gain broader application in non-rodent models.

This work was supported by ELKH-POC-2021-026 grant, the Lendület (“Momentum") Programme of the Hungarian Academy of Sciences to DH as well as by project no.129120 that has been implemented with the support provided by the Ministry of Innovation and Technology of Hungary from the National Research, Development and Innovation Fund, financed under the FK18 funding scheme from the Ministry for Culture and Innovation from the source of the National Research, Development and Innovation fund.

University

Semmelweis University

Supervisor

Daniel Hillier

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

8099

Start

15:25

End

15:18