PhD Scientific Days 2024

Poster Session A - Molecular Medicine 1.

Isolation and Characterisation of Extracellular Vesicles from Lymph Nodes

Előadó neve

Bodnár, Bernadett Réka

Neptun code

IC18JI

Előadó munkahelye

Department of Genetics, Cell- and Immunobiology, Semmelweis University

Előadó telefonszáma

+36300164493

Előadó e-mail címe

bodnar.bernadett.95@gmail.com

Az előadás címe

Isolation and Characterisation of Extracellular Vesicles from Lymph Nodes

Szerző(k) neve és munkahelye

Bernadett Réka Bodnár¹, Brachyahu Meir Kestecher¹, Sayam Ghosal¹, Edit Irén Buzás¹, Xabier Osteikoetxea¹

1: HCEMM-SU Extracellular Vesicles Research Group, Budapest, Hungary Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary

Bemutatás módja

Poszter

Szekció

Poster Session A - Molecular Medicine 1.

Language of the presentation

English

Preferred session

Mental Health Sciences

Összefoglaló szövege

Bernadett Réka Bodnár 1,2, Brachyahu Meir Kestecher *1,2,3, Sayam Ghosal 1,2, Edit Irén Buzás1,2,3, Xabier Osteikoetxea1,2
* Presenting author

Affiliation details:
1 HCEMM-SU Extracellular Vesicles Research Group, Budapest, Hungary Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary
2 Institute of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary
3 HUN-REN-SU Translational Extracellular Vesicle Research Group, Budapest

Introduction:
Extracellular vesicles (EVs) are lipid membrane-enclosed small particles that are released into the extracellular environment by all cells in nature. EVs can be obtained from cell conditioned media, biofluids and from the interstitial space of primary tissues. EV isolation from lymph nodes still remains a challenge, despite the well-described involvement of EVs in immune processes.

Aim:
Our aim here is to carry out the isolation and characterization of EVs from mouse lymph nodes.

Methods and Result:
C57BL/6 male mice were immunised with a stable emulsion of Complete Freund's Adjuvant alone or in combination with ovalbumin.
The inguinal and popliteal lymph nodes were isolated on day 9. EVs were separated from lymph nodes using differential centrifugation combined with size-exclusion chromatography and were further characterised using standard EV methodologies following the MISEV 2023 guidelines. Particle concentration was determined with Nanoparticle Tracking Analysis. Total protein concentration of the EV preparations was assessed with BCA assay and the total lipid content was measured with the SPV lipid assay. EV markers were analysed with a CytoFlex S Flow Cytometer. Within the large- and small-sized EV populations, we detected CD9, CD63 and Annexin V EV surface markers.

Conclusion:
Overall, here we present the isolation and characterisation EVs from the solid tissue of lymph nodes and the analysis of the effect immunisation on the isolated vesicles. Also, this work allows for further studies to understand immune processes and provides an opportunity to better understand the relationship between EVs and immune processes.

Funding:
This work was funded by the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement No 739593 and the Hungarian scientific research fund (OTKA) grant No FK147023

University

Semmelweis University

Supervisor

Xabier Osteikoetxea

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

8067

Start

15:45

End

15:48