PhD Scientific Days 2024

Molecular Medicine III.

The Effect of Extracellular Vesicles Originated from Mesenchymal Cells of Peritoneal Dialysate on the Mechanism of Fibrosis

Előadó neve

Bokrossy, Péter, MSc

Neptun code

G93AWK

Előadó munkahelye

Pediatric Center, MTA Center of Excellence, Semmelweis University, Budapest, Hungary

Előadó telefonszáma

+36708667254

Előadó e-mail címe

bokrossy.p@gmail.com

Az előadás címe

The Effect of Extracellular Vesicles Originated from Mesenchymal Cells of Peritoneal Dialysate on the Mechanism of Fibrosis

Szerző(k) neve és munkahelye

Péter Bokrossy¹, Beáta Szebeni¹, Apor Veres-Székely¹, Domonkos Pap¹, Zoltán Varga², Judith Mihály², Éva Pálinger³, Mária Bernáth⁴, György S. Reusz⁴, Attila J. Szabó¹, Ádám Vannay¹

1: Pediatric Center, MTA Center of Excellence, Semmelweis University, Budapest, Hungary; HUN-REN–SU Pediatrics and Nephrology Research Group, Budapest, Hungary
2: TTK Biological Nanochemistry Research Group, Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Budapest, Hungary
3: Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary
4: Pediatric Center, MTA Center of Excellence, Semmelweis University, Budapest, Hungary

Bemutatás módja

Szóbeli

Szekció

Molecular Medicine III.

Language of the presentation

English

Preferred session

Molecular Medicine

Összefoglaló szövege

Introduction: The literature is abundant on the topic of benefits of cell therapy in different experimental fibrosis models. Using extracellular vesicles (EVs) as an alternative to cell therapy promises benefits like lower immunogenicity, a possible crossing of the blood-brain barrier, and not inducing acute immune rejection.
Aims: We investigated the effect of EVs originating from mesenchymal cells (MCs) of peritoneal dialysate (PDE) on the activation of primary MCs and fibroblasts.
Methods: MCs were isolated, characterized, and cultured from PDE. From the serum-free cultures supernatant EVs were isolated by tangential flow filtration and size exclusion chromatography. After the isolation EVs were characterized based on their particle number, size distribution, morphological features, surface markers, and the composition of cargo proteins. Their effect on fibroblast activation was tested by in vitro experiments on primary peritoneal fibroblasts (pFBs) isolated from peritoneal biopsy collected during the removal of the Tenchoff catheter. The effects of EVs on the pFBs were examined by using functional assays such as MTT proliferation assay, Sirius Red assay, and Transient Agarose Spot assay.
Results: The mesenchymal cells isolated in the study displayed positive expressions of CK-18, α-SMA, CD73, CD105, and CD90 while lacking the CD34, HLA-DR, CD45, and CD19 markers as indicated by immunofluorescent staining and RT-PCR analysis. The isolated EVs exhibited stem cell and CK18 positivity, implying that their source cells were MCs that had undergone mesothelial mesenchymal transition. The EVs were successfully internalized by pFBs and reduced their PDGF-induced proliferation, TGF-β induced collagen accumulation, and EGF-induced migration as shown by various functional assays.
Conclusion: Due to the potential antifibrotic properties exhibited by these extracellular vesicles (EVs), they could hold therapeutic promise. Nevertheless, further in vivo testing is required to substantiate this hypothesis.
Funding: NKFIH K-142728, K-131594; 2020-1-1-2-PIACI-KFI_2020-00021, Semmelweis University, TKP2021-EGA-24, TKP2021-EGA-31, RRF-2.3.1-21- 2022-00003; HUN-REN, ELKH-POC-2022-024, Development and Innovation Fund, ÚNKP-23-3-I-SE-36, ÚNKP-23-3-I-SE-42, ÚNKP-23-4-II-SE-29, ÚNKP-23-5-SE-15; Hungarian Academy of Sciences, János Bolyai Research Scholarship.

University

Semmelweis University

Supervisor

Ádám Vannay

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

8110

Start

16:45

End

16:55