PhD Scientific Days 2024

Poster Session E - Molecular Medicine 2.

Glucan binding protein C specific DNA Aptamer shows inhibitory potential

Előadó neve

Mr. Harkai, Ákos

Neptun code

PFFTUP

Előadó munkahelye

Institute of Biochemistry and Molecular Biology

Előadó telefonszáma

06302508238

Előadó e-mail címe

akos.harkai@gmail.com

Az előadás címe

Glucan binding protein C specific DNA Aptamer shows inhibitory potential

Szerző(k) neve és munkahelye

Ákos Harkai¹, Yoon Kee Beck¹, Anna Tory¹, Brigitta Kállai¹, Tamás Mészáros¹

1: Institute of Biochemistry and Molecular Biology

Bemutatás módja

Poszter

Szekció

Poster Session E - Molecular Medicine 2.

Language of the presentation

English

Preferred session

Molecular Medicine

Összefoglaló szövege

Biofilm as a microbial virulence factor is a widespread survival tool for most prokaryotic species. In many cases, biofilm-forming bacteria are protected from the prevailing conditions and can thus become resistant to various disinfection procedures and antimicrobial treatments.
The use of a panel of antibiotics is still the gold standard in healthcare, but certain pathogenic bacteria are able to rapidly adapt to these drugs and develop new survival strategies against them. As a result, there is a need to develop effective biological molecules that can provide specific and long-lasting protection against harmful strains. Although the inhibition of bacterial growth by blocking biofilm formation is a relatively new frontier, the available circular peptides, monoclonal antibodies and aptamers appear to be a promising approach for defence against bacterial pathogens.
The focus of our research is the selection and application of aptamers specific for the cell surface protein GbpC of Streptococcus mutans UA130. GbpC plays a cardinal role in the formation of streptococcal biofilms, thereby promoting the development of dental caries. Specific inhibition of GbpC target with an aptamer can help to eliminate this important virulence factor, thereby mitigating and preventing oral diseases.
Based on our results, we succeeded in selecting GbpC-specific DNA aptamers, which was confirmed by demonstrating the GbpC-aptamer interaction by various methods. The applicability of the selected aptamers was tested in biofilm inhibition assays using wild-type and ΔGbpC S. mutans strains. With two candidate aptamers, we observed a significant biofilm reducing effect in the case of the wild-type but not in the ΔGbpC strain. We also investigated the diagnostic ability of the selected aptamers with our flow cytometric measurements.
Our future goal is to develop the efficacy of these aptamers by elucidating the atomic-level spatial structure of the aptamer-GbpC complex using X-ray crystallography.

University

Semmelweis University

Supervisor

Prof. Dr. Tamás Mészáros

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

8042

Start

14:45

End

14:48