PhD Scientific Days 2024

Pharmaceutical Sciences and Health Technologies II.

Development of liposomal corticosteroids

Előadó neve

Dr. Budavári, Bálint

Neptun code

OUC5ML

Előadó munkahelye

Department of Biophysics and Radiation Biology

Előadó telefonszáma

+36309562776

Előadó e-mail címe

budavari.balint.peter@semmelweis.hu

Az előadás címe

Development of liposomal corticosteroids

Szerző(k) neve és munkahelye

Bálint Budavári¹, Dr. S. Nagy Krisztina¹, Dr. Jedlovszky-Hajdú Angéla¹

1: Department of Biophysics and Radiation Biology

Bemutatás módja

Szóbeli

Szekció

Pharmaceutical Sciences and Health Technologies II.

Language of the presentation

English

Preferred session

Pharmaceutical Sciences and Health Technologies

Összefoglaló szövege

Introduction: Liposomes offer many advantages as nanoscale drug delivery systems regarding several pharmaceutical aspects. Since corticosteroids are lipophilic, they integrate into the lipid bilayer. This novel approach can improve the efficacy of several anti-inflammatory therapies.
Aims: Our goals were to create liposomes with long-term stability and the capability of transporting and releasing corticosteroids at inflamed body temperature. Considering the possible future application in asthma therapy, we also aimed at reaching high entrapment efficacy and slight drug leakage of the vesicles.
Methods: Liposome samples with two compositions were prepared from 3 different types of phospholipids in combination with Prednisolone (Pred) and budesonide (Bud) by the commonly used thin layer hydration method followed by extrusion to get small unilamellar vesicles (SUVs). Dynamic light scattering was applied for stability tests regularly and size-exclusion gel chromatography for the determination of drug entrapment efficacy. The effect of liposomal drugs on cell viability was assessed on the EBC-1 human lung carcinoma cell line. The combination of flow cytometry and fluorescence microscopy was applied to study the internalization of liposomes.
Results: SUVs with 100-120 nm in hydrodynamic diameter were successfully prepared. Their size-distribution has remained in the desired range through several months regarding most of the samples. In terms of stability, the presence of the corticosteroids was found to be more important than their type. The entrapment efficiency was between 70-85% and the drug leakage was 35-40 % for Pred and 6-8 % for Bud in the first 30 min. Neither the free corticosteroids nor their liposomal form affected significantly the cell viability. Both the microscopy images and flow cytometry results proved the internalization of the vesicles by the cells.
Conclusion: We have successfully created corticosteroid-loaded liposomes with long-term stability (6 months) and excellent entrapment efficiency due to the lipophilic character of the applied drugs. Furthermore, these liposomes are not toxic and can be internalized by the investigated pulmonary cells.
Funding:
Semmelweis 250+ Excellence PhD Scholarship, EFOP-3.6.3-VEKOP-16-2017-00009, NKFI FK 137749, TKP2021-EGA-23

University

Semmelweis University

Supervisor

Dr. Simonné Dr. Nagy Krisztina

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

6089

Start

09:45

End

09:55