PhD Scientific Days 2024

Poster Session H - Theoretical and Translational Medicine 1.

Comparative transcriptomic analysis of mouse models of heart failure induced by chronic angiotensin-II infusion or transverse aortic constriction

Előadó neve

Dr. Váradi, Barnabás

Neptun code

BQ2IRJ

Előadó munkahelye

Department of Pharmacology and Pharmacotherapy

Előadó telefonszáma

+36209859832

Előadó e-mail címe

varadi.barnabas@semmelweis.hu

Az előadás címe

Comparative transcriptomic analysis of mouse models of heart failure induced by chronic angiotensin-II infusion or transverse aortic constriction

Szerző(k) neve és munkahelye

Barnabás Váradi¹, Sayour Nabil V.², Gergely Tamás G², É. Tóth Viktória², Ágg Bence², Kovács Tamás², Kucsera Dániel², Kovácsházi Csenger², Brenner Gábor B², Giricz Zoltán², Ferdinandy Péter², Varga Zoltán V²

1: Semmelweis Egyetem Farmakológiai és Farmakoterápiás Intézet
2: Department of Pharmacology and Pharmacotherapy

Bemutatás módja

Poszter

Szekció

Poster Session H - Theoretical and Translational Medicine 1.

Language of the presentation

English

Preferred session

Theoretical and Translational Medicine

Összefoglaló szövege

Introduction: Heart failure with reduced ejection fraction (HFrEF) is a significant problem to public health due to its high mortality and prevalence. In order to bridge the gap between preclinical findings and clinical applications, it requires well-established, easy-to-perform in vivo preclinical animal models. However, the current gold standard model of HFrEF, the transverse aortic constriction (TAC) model, demands a team of highly skilled professionals and several weeks of follow-up for HFrEF to develop.
Aims: We aimed to investigate the feasibility of angiotensin II (Ang II) treatment-induced HFrEF model in Balb/c mice and to compare the model with the well-established TAC model at the molecular level.
Method: Balb/c mice were treated for 2 weeks with Ang II or vehicle with osmotic minipump, and C57BL/6J mice were treated with TAC or sham surgery. Echocardiography was performed to follow the cardiac function. After the sacrifice of the animals, heart samples were investigated by RNA sequencing in addition to basic pathology. Differential expression profiling was performed by the Hisat2-featureCounts-DESeq2 bioinformatics workflow. Differential expression changes in the two models were compared by correlation analysis with the use of Gene Ontology terms.
Results: Ang II treatment of Balb/c mice showed systolic cardiac dysfunction, but less ventricular dilatation and hypertrophy compared to the widely used TAC model. The observed transciprotmic changes were similar in general and in most of the investigated functions when comparing the two models.
Conclusion: We showed that chronic Ang II treatment of Balb/c mice is a relevant and reliable preclinical model for the study of HFrEF, which results very similar molecular changes in the heart compared to the TAC model, but it is much easier technically.
Funding: The research was supported by grants with following IDs: European Union's Horizon 2020 Research and Innovation Programme - no. 739593; a Momentum Research Grant - LP-2021-14 to ZVV; Project no. RRF- 2.3.1-21-2022-00003, European Union; NVKP_16-1-2016-0017 (“National Heart Program”); Thematic Excellence Programme - 2020-4.1.1.-TKP2020; Project no. TKP2021-EGA-23, TKP2021-EGA. FK134751; K_21-139105; EFOP-3.6.3-VEKOP-16-2017-00009.

University

Semmelweis University

Supervisor

Dr. Bence Ágg, Dr. Zoltán Varga

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

6872

Start

15:15

End

15:18