PhD Scientific Days 2024

Poster Session H - Theoretical and Translational Medicine 1.

Microbiota-gut-brain axis: fUS Detection of Inflammatory Connectome Changes

Előadó neve

Ms. Shailaja, Kanni Das

Neptun code

UG5346

Előadó munkahelye

Semmelweis University

Előadó telefonszáma

+36204779504

Előadó e-mail címe

kannidas@gmail.com

Az előadás címe

Microbiota-gut-brain axis: fUS Detection of Inflammatory Connectome Changes

Szerző(k) neve és munkahelye

Noémi Kovács¹, Ildikó Horváth¹, Zaira Zhaketay¹, Polett Hajdrik¹, Hajnal Márton¹, Krisztián Szigeti¹, Domokos Máthé¹, Kanni Das Shailaja¹

1: Semmelweis University

Bemutatás módja

Poszter

Szekció

Poster Session H - Theoretical and Translational Medicine 1.

Language of the presentation

English

Preferred session

Theoretical and Translational Medicine

Összefoglaló szövege

Aims: (a)To identify the different elements, constituents, and building blocks of microbial cells in the CNS and (b) To identify the measurable effects of these by imaging in vivo using different molecular imaging readouts in SPECT, functional ultrasound, and PET using an E. coli model bacteria system
Method: Iconeus One equipment was used for fUS. The anesthetized mouse was placed in a stereotaxic frame. Coupling gel was applied on the shaved surface of the mouse. Using Iconeus software, the probe was carefully positioned on the top of the ultrasound gel. First selected, the live view acquisition mode followed by the angiographic 3D scan option. Next, we selected the hippocampus and the striatum as landmarks of brain imaging. We studied the effect of LPS administration on the brain using fUS.
Results: Angio 3D scan is automatically registered to a reference template of mouse brain. Three views show the superposition of the vasculature and the mouse brain Allen atlas in the coronal, sagittal, and axial views. We observed changes in hippocampus region of the brain after LPS challenge
Conclusion: fUS is suitable technique to study the LPS induced changes in the brain.
Funding: European Union under project H2020-SmartAge grant Nr. 859890.

University

Semmelweis University

Supervisor

Domokos Máthé

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

7482

Start

15:20

End

15:23