PhD Scientific Days 2024

Pathological and Oncological Sciences II.

Bone Marrow-Derived Extracellular Vesicles from Irradiated Mice Are Able To Induce Senescence in Distinct Subpopulations of the Stem Cell Compartment and the Hematopoietic Microenvironment

Előadó neve

Ms. Forgács, Martina

Neptun code

AZ7M84

Előadó munkahelye

National Centre for Public Health and Pharmacy

Előadó telefonszáma

06306257203

Előadó e-mail címe

f.martina97@gmail.com

Az előadás címe

Bone Marrow-Derived Extracellular Vesicles from Irradiated Mice Are Able To Induce Senescence in Distinct Subpopulations of the Stem Cell Compartment and the Hematopoietic Microenvironment

Szerző(k) neve és munkahelye

Martina Forgács¹, Katalin Balázs¹, Ilona Barbara Csordás¹, Eszter Szarka¹, Balázs Gyebrovszki¹, Katalin Lumniczky¹

1: National Centre for Public Health and Pharmacy, Department of Radiobiology and Radiohygiene, Unit of Radiation Medicine, Budapest, Hungary

Bemutatás módja

Szóbeli

Szekció

Pathological and Oncological Sciences II.

Language of the presentation

English

Preferred session

Pathological and Oncological Sciences

Összefoglaló szövege

Introduction: Bone marrow (BM) stroma is a special microenvironment which has a strong influence on the function of the stem cell compartment. Radiation exposure can induce cellular senescence in specific cell types via dysregulation of specific molecular pathways. Previous studies (Kis et al, 2022) demonstrated that extracellular vesicles (EVs) from irradiated mice influence the cellular composition of the BM in the acute phase after irradiation.

Aims: The aim of this project is to better understand radiation-induced alterations and the role of the EVs released by the irradiated cells, by a long-term follow-up of phenotypical changes and senescence development in specific BM and stromal cell subpopulations.

Method: Male CBA/Ca mice were (I) directly irradiated with either 0Gy (controls) or 3Gy X-rays; or (II) injected with BM-derived EVs isolated from directly irradiated animals; or (III) both irradiated and injected with EVs. Long bones were isolated 3, 6 and 9 months after treatment. Single cell suspensions were prepared separately from the BM and bone. Major cell subpopulations within the bone and BM and B-gal staining indicative of cellular senescence in the respective cell subpopulations were investigated by flow cytometry.

Results: Ionizing radiation induced high level of cellular senescence in specific BM and stromal cell subpopulations, especially in those localized in the stromal environment. As a result of EV treatment the level of the cellular senescence in the mesenchymal stem cells and endothelial cells decreased in the BM and increased in the stroma during the follow-up, while in the case of fibroblast decreased the level of cellular senescence in the two system.

Conclusion: In conclusion, we showed that, not only ionizing radiation can cause abnormalities and trigger senescence in specific BM and stromal cell types, but also BM-derived EVs isolated from directly irradiated animals can induce bystander effect in certain stromal subpopulations in the recipient mice for a long-term period.

Reference: Kis, D., et al. Extracellular Vesicles Derived from Bone Marrow in an Early Stage of Ionizing Radiation Damage Are Able to Induce Bystander Responses in the Bone Marrow. Cells 2022, 11, 155. https://doi.org/10.3390/ cells11010155

University

Semmelweis University

Supervisor

Katalin Lumniczky, MD, PhD

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

7991

Start

17:15

End

17:25