PhD Scientific Days 2024

Poster Session H - Theoretical and Translational Medicine 1.

Inorganic Salt-Polysuccinimide Composite Scaffold for Potential Wound Dressing Application

Előadó neve

Ms. Pálos, Veronika, MSc

Neptun code

F9IEVV

Előadó munkahelye

Laboratory of Nanochemistry, Department of Biophysics and Radiation Biology, Semmelweis University

Előadó telefonszáma

+36303467269

Előadó e-mail címe

palos.veronika@phd.semmelweis.hu

Az előadás címe

Inorganic Salt-Polysuccinimide Composite Scaffold for Potential Wound Dressing Application

Szerző(k) neve és munkahelye

Veronika Pálos¹, Dorottya Gréta Kis¹, Krisztina S. Nagy¹, Rita Pázmány¹, Krisztina Juriga-Tóth¹, Bálint Budavári¹, Judit Domokos², Dóra Szabó², Ákos Zsembery³, Angéla Jedlovszky-Hajdú¹

1: Laboratory of Nanochemistry, Department of Biophysics and Radiation Biology, Semmelweis University
2: Institute of Medical Microbiology, Semmelweis University
3: Department of Oral Biology, Semmelweis University

Bemutatás módja

Poszter

Szekció

Poster Session H - Theoretical and Translational Medicine 1.

Language of the presentation

Hungarian

Preferred session

Theoretical and Translational Medicine

Összefoglaló szövege

Silver is increasingly being pushed out of medicine, and other ions with antibacterial effects are coming to the forefront. This is because silver ions have a huge environmental burden.
Our research aims to create a bicomponent polymer scaffold by electrospinning, which contains zinc and strontium salts in addition to the polymer, which, according to the literature, has antibacterial properties. If such a new type of biocompatible wound dressing could be created, it would be a mechanical barrier and have antibacterial activity against microorganisms.
Several experiments have been conducted to optimize the physicochemical, mechanical, and biological properties of the scaffolds developed for application as wound dressings. The first step was to synthesize the polysuccinimide (PSI) polymer, mix it with the selected inorganic salts in dimethylformamide, and optimize the electrostatic fiber formation parameters. The chemical and mechanical properties of the complete polymer networks were investigated by FTIR spectroscopy and SEM images, and their mechanical behavior through specific load capacity, elongation at breakpoint, and Young’s modulus value. We have also made dissolution tests to examine whether the salts could dissolve from the scaffold to exert their antibacterial effect. After that, antibacterial activity tests were performed on four application-relevant bacterial species. The next step was determining if the polymer scaffolds with the salts have cytotoxic activity against human tumor and fibroblast cells.
We proved that the salts are in the scaffolds and can dissolute from them to exert their antibacterial effect. Adding salts to the polymer changed the fiber diameters and mechanical properties. Except for the zinc acetate salt-containing ones, the scaffolds are not at all cytotoxic either to tumor or healthy cells.
We did further research to combine the two salts' advantageous properties. Coaxial fibers are nanofibers that have a core and a shell layer. We aim to make an inner core layer that contains the two salts and PSI and an outer shell layer that includes the neat PSI to get a more extended dissolution profile. First, the optimization of the coaxial electrospinning and mechanical characterization of the coaxial fibers has been carried out.

This research was supported by NKFIH FK 137749 and TKP2021-EGA-23 research grants.

University

Semmelweis University

Supervisor

Angéla Jedlovszky-Hajdú

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

8058

Start

15:30

End

15:33