PhD Scientific Days 2024

Molecular Medicine II.

GDF-15 and mtDNA deletions show potential as effective biomarkers for detecting mitochondrial dysfunction in cases of insulin resistance and PCOS

Előadó neve

Dr. Varhegyi, Vera

Neptun code

ZF3KHN

Előadó munkahelye

Semmelweis University, Department of Obstetrics and Gynecology / Semmelweis University, Institute of Genomic Medicine and Rare Disorders

Előadó telefonszáma

+36206663493

Előadó e-mail címe

varhegyi.vera@semmelweis.hu

Az előadás címe

GDF-15 and mtDNA deletions show potential as effective biomarkers for detecting mitochondrial dysfunction in cases of insulin resistance and PCOS

Szerző(k) neve és munkahelye

Vera Várhegyi¹, Anna Modos¹, Domonkos Trager², Dora Gerszi³, Eszter Maria Horvath⁴, Miklos Sipos³, Nandor Acs³, Maria Judit Molnar², Szabolcs Varbiro⁵, Aniko Gal²

1: Semmelweis University, Institute of Genomic Medicine and Rare Disorders / Semmelweis University, Department of Obstetrics and Gynecology
2: Semmelweis University, Institute of Genomic Medicine and Rare Disorders
3: Semmelweis University, Department of Obstetrics and Gynecology
4: Semmelweis University, Department of Physiology
5: Semmelweis University, Department of Obstetrics and Gynecology / University of Szeged, Albert Szent-Györgyi Health Centre, Department of Obstetrics and Gynecology

Bemutatás módja

Szóbeli

Szekció

Molecular Medicine II.

Language of the presentation

Hungarian

Preferred session

Molecular Medicine

Összefoglaló szövege

Introduction: There is no existing literature regarding the combination of the growth differentiation factor-15 (GDF-15) biomarker and mitochondrial DNA (mtDNA) deletions in insulin resistance (IR), polycystic ovary syndrome (PCOS), and the associated infertility. However, exploring this combination could prove valuable in achieving optimal metabolic health and enhancing pregnancy outcomes.
Aim: This study aimed to investigate the roles of GDF-15 and mtDNA deletions as biomarkers in the pathogenesis of IR and PCOS.
Patients and Methods: A total of 81 female patients diagnosed with IR, PCOS, or both, along with infertility and clinical signs indicating mitochondrial dysfunction, were examined, alongside 41 healthy controls. Blood and urine epithelial cells were isolated for DNA extraction. GDF-15 levels were measured using an ELISA kit, while mtDNA deletions were assessed through long-range PCR.
Results: GDF-15 levels in patients showed a significant elevation compared to controls. Specifically, 12 patients with elevated GDF-15 levels all had a BMI over 25 kg/m2 and exhibited reactive hyperinsulinemia. MtDNA deletions were observed in 61.5% of the patient group, predominantly among those with IR only. The increase in plasma GDF-15 levels and prevalence of mtDNA deletions were directly correlated with BMI. Patients with more pronounced metabolic abnormalities required more intensive drug therapy, leading to a parallel increase in plasma GDF-15 levels.
Conclusion: elevated GDF-15 levels and the presence of mtDNA deletions may stem from carbohydrate metabolism disorders in patients, potentially serving as indicators of an accelerated aging process.

Funding: This study was supported by NKFIH_FK_132812, János Bolyai Research Scholarship, ÚNKP-22-5 Research Scholarship (New National Excellence Program of the Ministry for
Innovation and Technology from the source of the National Research, Development and Innovation Fund) and TKP2021-EGA.25, TKP2021-NVA-15, SE STIA-OTKA-2021 and Hungarian
Society of Hypertension grants.

University

Semmelweis University

Supervisor

Aniko Gal

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

6907

Start

17:15

End

17:25