PhD Scientific Days 2024

Poster Session I - Neurosciences 2.

Noxious Stimulus-Responsive Neurons In The Ventral PAG and Dorsal Raphe Nucleus

Előadó neve

Mr. Földi, Péter

Neptun code

CZAEWS

Előadó munkahelye

HUN-REN Institute of Experimental Medicine

Előadó telefonszáma

+36306955348

Előadó e-mail címe

foldi.peter@koki.hun-ren.hu

Az előadás címe

Noxious Stimulus-Responsive Neurons In The Ventral PAG and Dorsal Raphe Nucleus

Szerző(k) neve és munkahelye

Péter Földi^1,2, Kinga Müller¹, Norbert Hájos¹

1: HUN-REN - Institute of Experimental Medicine, 'Lendület' Laboratory of Network Neurophysiology
2: Semmelweis University, János Szentágothai Doctoral School of Neurosciences

Bemutatás módja

Poszter

Szekció

Poster Session I - Neurosciences 2.

Language of the presentation

English

Preferred session

Neurosciences

Összefoglaló szövege

Introduction
The ventral periaqueductal grey (vPAG) including the ventrolateral PAG and dorsal raphe nucleus plays a critical role in controlling anxiety, fear memory formation, autonomic processes and most particularly, it is involved in descending modulation of pain processing. It has been shown that different neuron types, such as dopaminergic and serotonergic cells are part of this circuitry – besides the glutamatergic and GABAergic neurons – however their exact functions remained unclear. Additionally, malfunctioning of the circuit operation in the vPAG contributes to several neuropsychiatric disorders, the treatment of which is still a great challenge. Therefore, understanding the functional properties of neurons in this region can be critical in proposing new therapeutic approaches.
Aims
Here, we explored the single-unit activity in the vPAG in urethane-anesthetized mice in response to different types of noxious and neutral stimuli by applying two different recording approaches.
Methods
First, we performed acute silicone probe recordings to determine how neurons respond to different type of stimulations. Additionally, using the juxtacellular recording technique with the same set of external stimuli and post hoc immunocytochemistry to identify the recorded cell types, we distinguished functionally different neuron types in the vPAG.
Results
Analysing the firing features and neurochemical content of the recorded neurons, we found that dopaminergic neurons can be separated into two groups based on their response latency and vasoactive intestinal polypeptide content, suggesting their different involvement in noxious stimulation processing. Further, we revealed that serotonergic neurons are heterogeneous and can be clustered into five groups based on their responses upon noxious stimulation. Based on our observations serotonergic and dopaminergic neurons were exclusively responsive to non-neutral stimuli.
Conclusions
Our current results show that the firing of the monoaminergic neurons in the vPAG circuitries is distinctly modified by noxious stimuli, implicating their different contribution to pain processing in this clinically important brain region.

University

Semmelweis University

Supervisor

Dr. Norbert Hájos

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

8080

Start

15:15

End

15:18