PhD Scientific Days 2024

Poster Session F - Molecular Medicine 3.

ARHGAP25: A New Player in T Cell Physiology

Előadó neve

Dr. Sasvári, Péter

Neptun code

BEH4Y8

Előadó munkahelye

Department of Physiology, Semmelweis University

Előadó telefonszáma

06309650396

Előadó e-mail címe

sasvari.peter@phd.semmelweis.hu

Az előadás címe

ARHGAP25: A New Player in T Cell Physiology

Szerző(k) neve és munkahelye

Dr. Sasvári Péter¹, Dr. Csépányi-Kömi Roland¹

1: Department of Physiology, Semmelweis University

Bemutatás módja

Poszter

Szekció

Poster Session F - Molecular Medicine 3.

Language of the presentation

English

Preferred session

Molecular Medicine

Összefoglaló szövege

Introduction
ARHGAP25 is a Rac-specific GTPase activating protein playing a key role in immunological processes. Its function in neutrophilic granulocytes has been thoroughly elucidated, followed by the discovery of its importance in B lymphocyte biology. However, our current understanding of its function in T-cell biology is still limited.
Aims
We aim to investigate the role of ARHGAP25 on T cell maturation and functions, searching for novel ways to combat inflammatory and autoimmune diseases.
Method
We used healthy adult Arhgap25+/+ (wild type, WT) and Arhgap25-/- (knock-out, KO) mouse strains with C57BL/6 background, comparing T cell levels in peripheral blood, along with spleen, inguinal and axillary lymph node and thymus sizes and cellular compositions. T cells isolated from healthy spleens were cultured in RPMI-1640 medium to assess their viability and cellular functions.
Results
Peripheral blood of KO mice showed decreased CD3+ T cells with no change in CD4/CD8 ratios. Similar differences were detected in peripheral lymph nodes, resulting in a smaller size. The spleen showed no difference, but the thymus was significantly enlarged, with higher thymocyte counts and altered subpopulation ratios.
Conclusion
Apart from being a key player in innate immune functions, ARHGAP25 acts as a regulatory molecule in T cell development. The lack of ARHGAP25 protein disturbs healthy T-cell maturation and causes unusual thymus enlargement with a decreased T-cell count in the blood.
Funding
TKP2021-EGA-24, OTKA FK_128376

University

Semmelweis University

Supervisor

Dr. Csépányi-Kömi Roland

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

6060

Start

15:15

End

15:18