PhD Scientific Days 2024

Poster Session B - Pharmaceutical Sciences and Health Technologies 1.

Development of Levofloxacin-loaded Electrospun Nanofibrous Ocular Insert for the Treatment of Antibacterial Conjunctivitis

Előadó neve

Mr. Abboud, Houssam, CSc

Neptun code

IWD5AR

Előadó munkahelye

Semmelweis university

Előadó telefonszáma

704027555

Előadó e-mail címe

houssam.abboud@phd.semmelweis.hu

Az előadás címe

Development of Levofloxacin-loaded Electrospun Nanofibrous Ocular Insert for the Treatment of Antibacterial Conjunctivitis

Szerző(k) neve és munkahelye

Houssam Abboud¹, Eszter Farkas¹, Nándor Nagy¹, Balázs Pinke3², Romána Zelkó1¹, Adrienn Kazsoki1¹

1: Semmelweis University
2: Budapest University of Technology and Economics,

Bemutatás módja

Poszter

Szekció

Poster Session B - Pharmaceutical Sciences and Health Technologies 1.

Language of the presentation

English

Preferred session

Pharmaceutical Sciences and Health Technologies

Összefoglaló szövege

Introduction: Ocular inserts are significant candidates used to overcome the disadvantages of ophthalmic conventional dosage forms, particularly regarding infection treatment. Levofloxacin stands out among of the used drugs due to its wide antibacterial spectrum and low cytotoxicity, making it a crucial criterion for ocular inserts. Therefore, Levofloxacin can be a suitable option for formulations of ocular inserts.
Aims: This work aimed to prepare levofloxacin-containing, polyvinyl alcohol (PVA)--based ocular insert by electrospinning using different excipients. Moreover, to investigate how the different molecular weights of PVA and the other excipients affect the fibers' characteristics and the drug profile release.
Methods: PVA (Mowiol® 18-88 and 40-88) was chosen as the base of the electrospun samples. Poloxamer 407 was added as a mucoadhesive-enhancing agent. The total polymer concentration was set at 3 levels: 10, 12, and 14 % (w/w). Hydroxypropyl beta-cyclodextrin (HP-β-CD) was used in a 1:1, 1:1.5 mol ratio to increase the solubility of the drug. The morphological analysis of the samples was performed by Scanning Electron Microscopy and their solid phase characterization by Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD). The dissolution study was performed in phosphate buffer (pH 7.4). The drug concentration was determined by UV-vis spectroscopy.
Results: The morphological test confirmed the fibrous structure of all the produced samples. The FT-IR spectra showed the formulation of amorphous solid dispersion which was confirmed by X-ray diffraction measurements. The drug release from the nanofibrous samples was fast and complete. By increasing each cyclodextrin amount and PVA molecular weight, slightly prolonged (but also very fast) drug dissolution was achieved. In the case of the formulations of Mowiol® 18-88 eye irritation test was carried out on the chorioallantois membrane of fertilized eggs and showed eye irritation in the case of the 1:1,5 mol ratio of drug: CD.
Conclusion: The Mowiol® 18-88 based formulation with a 1:1 drug: HP-β-CD mol ratio could be suitable for the treatment of antibacterial conjunctivitis.
Funding: This project was supported by ÚNKP-23-4-II-SE-22 New National Excellence Program of the Ministry for Culture and Innovation from the source of the National Research, Development, and Innovation Fund.

University

Semmelweis University

Supervisor

Adrienn Kazsoki1

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

8020

Start

15:35

End

15:38