PhD Scientific Days 2024

Poster Session H - Theoretical and Translational Medicine 1.

A Systematic Interrogation of Splicing Events for Personalised Medicine (Molecular Subtyping) and Biomarker Candidates in Non-Muscle-Invasive Bladder Cancer (NMIBC)

Előadó neve

Yuksek Tekben, Esra Hilal, PhD

Előadó munkahelye

Institute of Cancer and Genomics Science

Előadó telefonszáma

07732237496

Előadó e-mail címe

exy263@student.bham.ac.uk

Az előadás címe

A Systematic Interrogation of Splicing Events for Personalised Medicine (Molecular Subtyping) and Biomarker Candidates in Non-Muscle-Invasive Bladder Cancer (NMIBC)

Szerző(k) neve és munkahelye

Esra Hilal Yuksek Tekben¹

1: Institute of Cancer and Genomics Science

Bemutatás módja

Poszter

Szekció

Poster Session H - Theoretical and Translational Medicine 1.

Language of the presentation

English

Preferred session

Theoretical and Translational Medicine

Összefoglaló szövege

IntroductionBladder cancer is the tenth most prevalent cancer worldwide and represents a significant cause of mortality. The prevalence of bladder cancer is increasing globally.Identification of isoforms differences between different grades of bladder cancer may result in the identification of markers of progression or prognosis. Thus, in the context of high and low grades of bladder cancer physiology, our understanding identified isoforms as well as other types of isoforms continues to grow substantially.
AimsThe objective of this project is to systematically investigate isoform ‘switching events’ as well as novel isoforms as an additional layer of information for patient stratification. I aim to identify isoform-based biomarkers and molecular subtypes in NMIBC using RNA sequencing data. After the systematic determination of isoform switching, differentially isoform expression analysis will be performed to determine isoformbased subtypes. Candidate isoform biomarkers identified from short-read sequencing will be validated with qPCR and focused long-read techniques.
MethodsBladder cancer patients were recruited from ten hospitals in the UK. RNA was extracted from tissue and blood samples. Then, RNA sequencing was performed on RNA extracted.
For an initial differential isoform expression analysis between bladder cancer samples, we adapt the differential gene expression workflow, albeit on isoform expression with Salmon.
In terms of statistics, eliminating low count genes makes it possible to estimate the mean-variance relationship more accurately in the data.
Results - I determined differentially expressed isoforms and significant switching isoforms between low and high grades of bladder cancer.
Conclusion-Our analysis successfully identified differentially expressed isoforms and significant switching events between low and high grades of bladder cancer. This finding sheds light on the molecular mechanisms of bladder cancer, potentially offering valuable insights into diagnostic strategies. By elucidating the dynamic changes in isoformexpression, our study contributes to a deeper understanding of bladder cancer biology and paves the way for further investigations into isoformlevel alterations as potential biomarkers and therapeut ictargets.
Funding- supported by the Ministry of National Education of Turkey.

University

University of Birmingham

Supervisor

Professor Rik Bryan, Dr. Roland Arnold

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

8238

Start

14:50

End

15:03