PhD Scientific Days 2024

Poster Session Q - Pathological and Oncological Sciences 2.

Differential effects of hypoxia on the motility of lung adenocarcinoma cell lines

Előadó neve

Surguta, Sára

Neptun code

pkw3mo

Előadó munkahelye

Országos Onkológiai Intézet

Előadó telefonszáma

06303672588

Előadó e-mail címe

surguta.sara@ext.oncol.hu

Az előadás címe

Differential effects of hypoxia on the motility of lung adenocarcinoma cell lines

Szerző(k) neve és munkahelye

S.E. Surguta¹, M. Baranyi², L. Svajda¹, I. Randelovic³, M. Cserepes³, J. Tóvári¹

1: Department of Experimental Pharmacology and National Tumor Biology Laboratory, National Institute of Oncology, Budapest, Hungary; Doctoral School of Semmelweis University, Budapest, Hungary
2: Department of Pathology, Forensic and Insurance Medicine, Semmelweis University, Budapest, Hungary
3: Department of Experimental Pharmacology and National Tumor Biology Laboratory, National Institute of Oncology, Budapest, Hungary

Bemutatás módja

Poszter

Szekció

Poster Session Q - Pathological and Oncological Sciences 2.

Language of the presentation

Hungarian

Preferred session

Pathological and Oncological Sciences

Összefoglaló szövege

Introduction: Metastasis formation is a common cause of mortality in lung cancer patients, and it is hypothesized that hypoxia plays a key role in this process. However, the impact of hypoxia on the metastatic potential and migratory activity of cancer cells is largely unexplored and requires detailed investigations.
Material and methods: In our study, we included lung adenocarcinoma cell lines carrying different mutations of the growth factor pathway (H1975: EGFR T790M, PF901: BRAF V600E, PF139: KRAS G12C mutant, and H838: triple WT). We analyzed the changes in cell proliferation and migration activity under normoxic and hypoxic (1% O2) conditions in both single-cell and collective migration using scratch assay and video microscopy experiments. We also examined changes in key genes and proteins related to the hypoxic response, epithelial-mesenchymal transition, proliferation, and apoptosis using Western Blot and qPCR techniques.
Results: Chronic hypoxia proved to be an acute stressor for the cells. Generally, reduced proliferation was observed under hypoxic conditions without detectable induction of apoptosis. Interestingly, we observed that single-cell motility was generally reduced, while collective migration was increased in most cell lines under hypoxia. Additionally, changes were noted in the expression of EMT markers, consistent with the enhanced motility and metastasis-promoting effects of hypoxia.
Conclusion: In summary, our study suggests that the effects of hypoxia on proliferation, motility, and gene expression were cell line-, exposure time-, and migration type-dependent. Our findings contribute to a better understanding and potential treatment of metastasis.
Funding: The authors acknowledge financial support from the National Laboratories Excellence program [under the National Tumor Biology Laboratory Project (NLP-17, 2022-2.1.1-NL-2022-00010] and the Hungarian Thematic Excellence Program [TKP2021-EGA-44]. Project no. 1018567 has been implemented with the support provided by the Ministry of Culture and Innovation of Hungary from the National Research, Development and Innovation Fund, financed under the KDP-2020 funding scheme.

University

Semmelweis University

Supervisor

Dr. József Tóvári

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

Előadó születési éve

1995

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

8340

Start

16:45

End

16:48