PhD Scientific Days 2024

Poster Session E - Molecular Medicine 2.

A C-type lectin receptor is indispensable for experimental bullosus skin disease

Előadó neve

Dr. Balogh, Lili

Neptun code

CMGK6E

Előadó munkahelye

Department of Physiology, Semmelweis University

Előadó telefonszáma

06 1 459 1500 / 60334

Előadó e-mail címe

balogh.lili@stud.semmelweis.hu

Az előadás címe

A C-type lectin receptor is indispensable for experimental bullosus skin disease

Szerző(k) neve és munkahelye

Lili Balogh^1,2, Eszter Káposztás^1,2, Silvia Hayer³, Stephan Blüml³, Attila Mócsai¹, Tamás Németh^1,2,4

1: Department of Physiology, Semmelweis University
2: MTA-SE “Lendület” Translational Rheumatology Research Group, Hungarian Academy of Sciences and Semmelweis University
3: Department of Rheumatology, Medical University of Vienna, Vienna, Austria
4: Department of Rheumatology and Clinical Immunology, Semmelweis University, Budapest, Hungary

Bemutatás módja

Poszter

Szekció

Poster Session E - Molecular Medicine 2.

Language of the presentation

English

Preferred session

Molecular Medicine

Összefoglaló szövege

Introduction: Dectin-2 polymorphisms were associated with rheumatoid arthritis ,while in immune complex-activated neutrophils the Dectin-2 gene showed a massive upregulation. These findings raised the possibility that Dectin-2 might have a role in autoimmune processes, for example in epidermolysis bullosa acquisita (EBA), which is a subepidermal blistering skin disease, which is triggered by autoantibodies against type VII collagen (CVII).
Aims: We investigated the role of Dectin-2 in a murine model of EBA.
Method: We used wild type and Dectin-2 knockout (Dectin‒/‒) mice in our experiments. Experimental EBA in mice was triggered by subcutaneous injections of anti-CVII. Disease progression was followed by clinical assessment and quantitative scoring. Ear thickness was measured with a caliper. To investigate the microscopic phenotype, histological H&E-stained sections and direct immunfluorescence samples were prepared. In vivo immune cell accumulation was detected by flow cytometry, while local chemokine levels and circulating anti-CVII antibodies were measured by ELISA.
Results: Dectin-2‒/‒ mice were almost completely protected from the experimental dermatitis in contrast to wild type animals, while the antibody-deposition and the serum anti-CVII levels did not differ in the two genotypes. The in vivo neutrophil and macrophage recruitment and the chemokine levels in the ears were also massively reduced in Dectin-2‒/‒ mice.
Conclusion: Our results indicate that a C-type lectin receptor Dectin-2 is essential for the development and progression of autoantibody-induced experimental dermatitis. Our findings can lead to a better understanding of the pathogenesis of autoimmune blistering skin diseases, which may lead to new therapies in the future. .
Funding: This work was supported by the Hungarian National Research, Development and Innovation Office.

University

Semmelweis University

Supervisor

Tamás Németh MD, PhD, habil

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

7444

Start

15:25

End

15:28