PhD Scientific Days 2025

Poster Session III. - H: Pharmaceutical Sciences and Health Technologies

Flagellin Pretreatment Ameliorates Indomethacin-induced Enteropathy in Mice

Előadó neve

Haghighi Arezoo, PhD

Neptun code

FHW40X

Előadó munkahelye

Department of Pharmacology and Pharmacotherapy

Előadó telefonszáma

06704202436

Előadó e-mail címe

arezoo.haghighi@phd.semmelweis.hu

Az előadás címe

Flagellin Pretreatment Ameliorates Indomethacin-induced Enteropathy in Mice

Szerző(k) neve és munkahelye

Arezoo Haghighi¹, Zsuzsanna O. Demeter¹, Anna Zsidai¹, Samaneh Haghighi², Gerda Wachtl¹, Klára Gyires¹, Zoltán S. Zádori¹

1: Department of Pharmacology and Pharmacotherapy
2: Institute of Translational Medicine, Semmelweis University, Budapest, Hungary

Bemutatás módja

Poszter

Szekció

Poster Session III. - H: Pharmaceutical Sciences and Health Technologies

Language of the presentation

English

Preferred session

Pharmaceutical Sciences and Health Technologies

Összefoglaló szövege

Introduction: Previous studies have shown that treatment with flagellin, the toll-like receptor 5 (TLR5) ligand, reduces gastrointestinal injury induced by various stimuli. In addition, we found that indomethacin (IND)-induced small intestinal injury (enteropathy) in rats is associated with downregulation of TLR5, and TLR5 expression negatively correlates with intestinal inflammation. Therefore, we hypothesised that pretreatment with flagellin can reduce IND-induced enteropathy.
Aim: To assess the protective effect of flagellin in two different doses against indomethacin-induced small intestinal damage.
Methods: Enteropathy was induced in mice by IND at a dose of 40 mg/kg. Flagellin was injected intraperitonelly 2 h before and 4 h after IND administration at 2 different doses (10 and 30 mg/mouse). Tissue levels of inflammatory proteins and TLRs were assessed by Western blot and qPCR.
Results: IND induced enteropathy after 24 h, characterised by intestinal ulceration and inflammation. Tissue levels of several inflammatory mediators and cytokines were elevated, including MPO, PTX3, COX2 and IL-1β. The ratio of Bax to Bcl-2 increased, indicating increased apoptosis. In addition, IND increased the gene expression of TLR2 and TLR4 and decreased that of TLR1, TLR5 and TLR9. Most of these changes were prevented by flagellin treatment, except for the downregulation of TLR5 expression.
Conclusion: Our study suggests that flagellin treatment may be a potential strategy to protect the small intestine against IND-induced enteropathy.
Grant: NKFI FK 138842, SE250+

University

Semmelweis University

Supervisor

Zoltán S. Zádori

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

phd.section.field

in doctoral studies after complex exam (PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

7340

Start

14:12

End

14:18