PhD Scientific Days 2025

Poster Session II. - J: Theoretical and Translational Medicine

Internal Dynamics of T7 bacteriophage Target Receptors

Előadó neve

Dr. Kiss Bálint

Neptun code

V3RJ08

Előadó munkahelye

Department of Biophysics and Radiation Biology

Előadó telefonszáma

06304642508

Előadó e-mail címe

onlybalint@gmail.com

Az előadás címe

Internal Dynamics of T7 bacteriophage Target Receptors

Szerző(k) neve és munkahelye

Bálint Kiss¹, Luca Kosik¹, Miklós Kellermayer¹, Hedvig Tordai¹, Dominik Sziklai¹, Miklós Cervenak¹

1: Department of Biophysics and Radiation Biology

Bemutatás módja

Poszter

Szekció

Poster Session II. - J: Theoretical and Translational Medicine

Language of the presentation

English

Preferred session

Theoretical and Translational Medicine

Összefoglaló szövege

Viruses have long been the focus of biological research, and due to the recent development of single-particle observation methods their structure and infection mechanisms have gained even more attention. T7 bacteriophages are icosahedral dsDNA viruses, which infect E. coli bacteria. Although this type of bacteriophage has been a workhorse of virus research, we still do not understand every step of its infection process. T7 virions are comprised of a protein shell encapsulating the viral DNA, and a tail-fiber complex which is primarily used for target recognition and DNA translocation across the host cell membrane. There are six viral fibers attached to the tail which are essential for initial virus to host attachment, as well as ideal phage orientation on the bacterial surface. Our recent research has focused on the structure and role of these tail-fibers. Using atomic force microscopy (AFM) we have been able to observe fibers in their native state, which were found to be bound on the capsid surface. Using high-speed AFM, we have observed loosely bound viral fibers on the capsid surface, which also revealed internal fiber dynamics. The “L” shaped fiber was found to be flexible; their distal regions were moving compared to the proximal regions, which hints at a more efficient, dynamic host recognition process. This observation was also confirmed by conventional AFM images of isolated viral fibers. The angle between the proximal and distal regions was found to be different from fiber to fiber (95.9 ± 12.7°, n=208). Our experiments hint at innate viral receptor dynamics, which may be unique to T7 bacteriophages, and allow them to better explore their host cell surface for a successful infection.

University

Semmelweis University

Supervisor

Miklós Kellermayer

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

phd.section.field

after finishing doctoral studies with absolutorium (PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4130

Start

18:00

End

18:06