PhD Scientific Days 2025

Poster Session II. - E: Pathological and Oncological Sciences

Galactose-induced metabolic adaptation and stem cell enrichment in small cell lung carcinoma cell lines

Előadó neve

Sztankovics Dániel, PhD

Neptun code

ZO54P8

Előadó munkahelye

Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary

Előadó telefonszáma

+36702579264

Előadó e-mail címe

sztankovics.daniel@semmelweis.hu

Az előadás címe

Galactose-induced metabolic adaptation and stem cell enrichment in small cell lung carcinoma cell lines

Szerző(k) neve és munkahelye

Dániel Sztankovics¹, Fatime Szalai¹, Dorottya Moldvai¹, Viktória Varga¹, Titanilla Dankó¹, Ágnes Czeti¹, Gábor Szalóki¹, Gábor Barna¹, Katalin Mészáros², Ildikó Krencz¹, Anna Sebestyén¹

1: Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary
2: Department of Laboratory Medicine, Semmelweis University, Budapest, Hungary

Bemutatás módja

Poszter

Szekció

Poster Session II. - E: Pathological and Oncological Sciences

Language of the presentation

Hungarian

Preferred session

Pathological and Oncological Sciences

Összefoglaló szövege

Introduction. There have been no major advances in treating small cell lung carcinoma (SCLC) in recent decades, and platinum-based chemotherapy remains the cornerstone of therapy. Like other malignancies, SCLC is characterised by high intratumoral heterogeneity, which significantly contributes to developing therapeutic resistance and selecting resistant clones. A rare and heterogeneous subpopulation of tumour cells, known as cancer stem cells (CSCs) or quiescent cells, has been implicated in tumour progression, relapse, and metastasis. These cells exhibit chemoresistance, metastatic potential, and high metabolic plasticity.
Aims. Our work aimed to characterise the quantitative and metabolic changes in cells expressing CSC markers.
Methods. Human SCLC cell lines (H841, H196, H146) were cultured in vitro in glucose-depleted medium supplemented with galactose. Flow cytometry was used to monitor changes in the expression of CSC markers (CD24, CD44, CD133 expression, and aldehyde dehydrogenase 1 (ALDH1) activity) in response to alternative nutrient sources. The anti-proliferative effects of cisplatin, as well as various metabolic and mTOR inhibitors, were assessed using the alamarBlueTM (AB) and sulforhodamine B (SRB) assays. Liquid chromatography-mass spectrometry (LC-MS) was used to determine changes in intracellular metabolite concentrations.
Result. CD44 expression increased in the presence of galactose in all cell lines tested in vitro. Two cell lines also exhibited distinct subpopulations with stem cell markers: a CD133-positive population in H841 and a CD24/CD44 double-positive population in H196. In parallel, we also observed proliferative changes and metabolic rearrangements, shifting from the Warburg phenotype to the oxidative phosphorylation (OXPHOS) phenotype, associated with galactose consumption.
Conclusion. Our results suggest that galactose, as an alternative nutrient source, induces an OXPHOS-driven metabolic rearrangement in the investigated cell lines and promotes the enrichment of stem cell phenotypic features, facilitating the study of therapeutically resistant subpopulations in vitro.
Funding. TKP2021-EGA-24 [A.S.], NKFI-K-142799 [A.S.], NKFI-PD-146373 [I.K.], 2024-2.1.1-EKÖP-2024-00004 [D.S., F.S., D.M., V.V.], EFOP-3.6.3-VEKOP-16-2017-00009 [F.S., D.M., V.V.]

University

Semmelweis University

Supervisor

Anna Sebestyén

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

phd.section.field

after finishing doctoral studies with absolutorium (PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

6049

Start

18:00

End

18:06