PhD Scientific Days 2025
Budapest, 7-9 July 2025
Poster Session III. - K: Theoretical and Translational Medicine
Diagnostic Accuracy of Rapid Molecular Assays Versus Blood Culture for the Detection of Bloodstream Infections: A Systematic Review and Meta-Analysis
Előadó neve
Dr. Rapszky Gabriella Anna
Neptun code
pp4y65
Előadó munkahelye
Demartment of Emergency Medicine, Semmelweis Univeristy
Előadó telefonszáma
+36305742899
Előadó e-mail címe
rapszky.gabriella.anna@semmelweis.hu
Az előadás címe
Diagnostic Accuracy of Rapid Molecular Assays Versus Blood Culture for the Detection of Bloodstream Infections: A Systematic Review and Meta-Analysis
Szerző(k) neve és munkahelye
Gabriella Anna Rapszky1, Uyen Nguyen Do To2, Veronika Eszter Kiss1, Tamás Kói3, Anna Walter4, Dorottya Gergő5, Fanni Adél Meznerics6, Márton Rakovics7, Szilárd Váncsa8, Lajos Vince Kemény6, Dezső Csupor9, Péter Hegyi10, Michael R. Filbin11, Csaba Varga1, Bánk G. Fenyves1
1: Department of Emergency Medicine, Semmelweis University
2: András Pető Faculty, Semmelweis University
3: Budapest University of Technology and Economics, Department of Stochastics
4: Institute for Translational Medicine, Medical School, University of Pécs
5: Department of Pharmacognosy, Semmelweis University
6: Department of Dermatology, Venereology and Dermatooncology, Semmelweis University
7: Eötvös Loránd University, Faculty of Social Sciences, Department of Statistics
8: Institute of Pancreatic Diseases, Semmelweis University
9: Institute of Clinical Pharmacy, University of Szeged
10: Centre for Translational Medicine, Semmelweis University
11: Department of Emergency Medicine, Massachusetts General Hospital and Harvard Medical School
Bemutatás módja
Poszter
Szekció
Poster Session III. - K: Theoretical and Translational Medicine
Language of the presentation
Hungarian
Preferred session
Theoretical and Translational Medicine
Összefoglaló szövege
Introduction
Early targeted antibiotics are key to better outcomes in sepsis. Rapid molecular assays (RMAs) can detect bloodstream infections (BSIs) faster than blood cultures (BCs), but their accuracy isn’t well established. We reviewed the literature to assess how well commercial RMAs perform compared to BCs.
Aims
To evaluate the diagnostic performance of commercially available RMAs for rapid pathogen detection of BSI in whole blood, compared to BC.
Method
We performed a systematic review and meta-analysis of studies from MEDLINE, Cochrane Library, Embase, and Web of Science (inception-September 23, 2024). Included studies enrolled patients with suspected or confirmed BSI tested with both a RMA (≤12 h turnaround, ≥20 pathogen targets) and BC. Animal studies and non-original articles were excluded. Primary outcomes were pooled sensitivity and specificity of RMAs versus BC. Bivariate analysis generated summary receiver operating characteristic curves and diagnostic metrics, stratified by unit of analysis, RMA type, and patient population. Risk of bias was assessed using QUADAS-2 and QUADAS-C. The study was registered in PROSPERO (CRD42022377280).
Results
Of 63,916 records, 104 studies were included in the qualitative and 75 in the quantitative synthesis, covering 17,952 samples and 11,393 patients (analyzed separately). Eleven RMAs were identified: four were included in RMA-specific subgroup analysis (SeptiFast, IRIDICA, SepsiTest, MagicPlex), five in the pooled analysis (UMD-SelectNA, VYOO®, MicrobScan, MicrobScan-Kairos24/7, REBA Sepsis-ID), and two in the qualitative synthesis only (InfectID-BSI, Pilot Gene Technology ddPCR). Pooled specificity (0.858; 95% CI: 0.830–0.883) was higher than sensitivity (0.659; 95% CI: 0.594–0.719) by patient. RMA-based sensitivity ranged from 0.492 (MagicPlex) to 0.783 (IRIDICA), and specificity by patient population ranged from 0.811 (ICU) to 0.892 (ED). Similar results were observed by sample. All studies were judged at high risk of bias.
Conclusion
RMAs offer shorter turnaround times but lack the sensitivity required to replace BCs. However, they may add diagnostic value as add-on tests by increasing overall pathogen detection. Advancements in assay sensitivity and high-quality clinical studies are needed to better define their role in clinical practice.
Funding
Centre for Translational Medicine, Semmelweis University.
University
Semmelweis University
Other university, not listed above
-
Supervisor
Bánk G. Fenyves
Publication of my abstract
I give consent to the publication of my abstract on the website of the congress.
phd.section.field
in doctoral studies after complex exam (PhD)
Kind
Szabad
Status
elfogadva
Accepted presentation method
poszter
Előadás fájl jóváhagyás
nem rendelkezett róla
Előadó
8616
Start
15:00
End
15:06