PhD Scientific Days 2025
Budapest, 7-9 July 2025
Pharmaceutical Sciences and Health Technologies I.
Green HPLC Strategy for Chiral Resolution of Crizotinib on Polysaccharide-Based Columns
Előadó neve
Mhammad Ali, PhD
Neptun code
GFI24E
Előadó munkahelye
Department of Pharmaceutical Chemistry, Semmelweis University
Előadó telefonszáma
+36707754866
Előadó e-mail címe
ali.mhammad@phd.semmelweis.hu
Az előadás címe
Green HPLC Strategy for Chiral Resolution of Crizotinib on Polysaccharide-Based Columns
Szerző(k) neve és munkahelye
Ali Mhammad1,2, Gergely Dombi1,2, Máté Dobó1,2, Zoltán-István Szabó3,4, Gergő Tóth1,2
1: Department of Pharmaceutical Chemistry, Semmelweis University, Hőgyes E. u. 9, Budapest, 1092, Hungary
2: Center for Pharmacology and Drug Research & Development, Semmelweis University,Budapest, Hungary
3: George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, Târgu Mureş, Romania
4: Sz-imfidum Ltd, Lunga, 525401, Romania
Bemutatás módja
Szóbeli
Szekció
Pharmaceutical Sciences and Health Technologies I.
Language of the presentation
English
Preferred session
Pharmaceutical Sciences and Health Technologies
Összefoglaló szövege
Introduction: With the growing emphasis on environmentally friendly analytical
practices, green approaches to chiral separation—particularly high-performance liquid chromatography (HPLC) —have gained increasing attention as essential tools for determining the enantiomeric composition of chiral pharmaceutical compounds.
Aims: To develop a green and efficient HPLC method for the enantioseparation of
crizotinib, a tyrosine kinase inhibitor targeting anaplastic lymphoma kinase.
Methods: The enantioselective performance of seven polysaccharide-based chiral
stationary phases was evaluated, including three amylose-derived (Lux Amylose-1, Lux
i-Amylose-1, Lux Amylose-2) and four cellulose-derived columns (Lux Cellulose-1, Lux
Cellulose-2, Lux Cellulose-3, and Lux Cellulose-4), under both polar organic and
reversed-phase modes.
Results: Among these, Lux Cellulose-3, which incorporates cellulose tris(4-methylbenzoate) as the chiral selector, demonstrated the best resolution using an
ethanol–water mixture with 0.1% diethylamine as the mobile phase. Chromatographic
parameters (mobile phase composition, flow rate, and temperature) were optimized using a full factorial screening design. Under optimal conditions (Lux Cellulose-3 column, ethanol/water/diethylamine 92/8/0.1 v/v/v, 0.5 mL/min flow rate at 40 °C column temperature), baseline enantioseparation was achieved. The method was validated in accordance with current guidelines. The greenness of the proposed method was confirmed through Analytical Eco-Scale, Complex GAPI, and AGREE metrics, showing superior environmental performance compared to a previously published method.
Conclusion: These findings highlight the method's reduced solvent hazards and waste generation, supporting its classification as a green analytical procedure.
Fundings: This work was funded by the National Research, Development, and Innovation Office, Hungary (grant: NKFIH FK 146930).
University
Semmelweis University
Other university, not listed above
no
Supervisor
Gergő Tóth
Publication of my abstract
I give consent to the publication of my abstract on the website of the congress.
phd.section.field
in doctoral studies before complex exam (PhD)
Kind
Szabad
Status
elfogadva
Accepted presentation method
szóbeli
Előadás fájl jóváhagyás
jóváhagyta
Előadó
8926
Start
14:45
End
15:00