PhD Scientific Days 2025

Poster Session I. - F: Pharmaceutical Sciences and Health Technologies

Comprehensive Characterization of Phytochemical Composition, Membrane Permeability, and Antiproliferative Activity of Juglans nigra Polyphenols

Előadó neve

Dr. Osztie Rita

Neptun code

IT24UX

Előadó munkahelye

Department of Pharmacognosy

Előadó telefonszáma

06303541199

Előadó e-mail címe

osztie.rita@stud.semmelweis.hu

Az előadás címe

Comprehensive Characterization of Phytochemical Composition, Membrane Permeability, and Antiproliferative Activity of Juglans nigra Polyphenols

Szerző(k) neve és munkahelye

Dr. Rita Osztie¹

1: Department of Pharmacognosy

Bemutatás módja

Poszter

Szekció

Poster Session I. - F: Pharmaceutical Sciences and Health Technologies

Language of the presentation

Hungarian

Preferred session

Pharmaceutical Sciences and Health Technologies

Összefoglaló szövege

Introduction: Juglans species are rich sources of bioactive natural products, cytotoxic activity of constituents isolated from walnut species has also been described. However, the comprehensive screening of the phenolic profile of J. nigra has not been performed yet.
Aims: The aim of our study was the detailed polyphenol profiling of Juglans nigra and the characterization of the membrane permeability and antiproliferative properties of its main isolated phenolics.
Methods: We prepared extracts of different plant parts of J. nigra using solvents with different polarities, and analyzed their phenolic composition using UHPLC-Orbitrap-MS. The isolation of the compounds was performed by flash chromatography and reversed-phase semi-preparative HPLC methods. The structures of the isolated compounds were identified by HR-MS and NMR spectroscopy. The in vitro antiproliferative activity was determined by Alamar Blue assay in three human cancer cell lines. The passive diffusion of the compounds across biological membranes was determined using in vitro models (PAMPA-BBB and PAMPA-GI).
Results: We have performed a comprehensive phytochemical screening of J. nigra leaf, bark, and pericarp samples. A total of 161 compounds were tentatively identified: flavonoids, phenolcarboxylic acids, ellagitannins, and naphthoquinones. Additionally, we described several gallotannins, flavonoids, juglone, and tetralone derivatives, and linear and cyclic diarylheptanoids in J. nigra for the first time. Eight compounds, including three flavonol-O-glycosides, juglone, 1,2,3,4-tetrahydro-7,8-dihydroxy-4-oxonaphthalen-1-yl-6-O-galloyl-glucoside, ellagic acid, gallic acid, and ethyl gallate, were isolated from J. nigra pericarp. The in vitro antiproliferative activity of the isolated compounds confirmed that juglone inhibits cell proliferation, and has similar activity as the clinical standards.
Both juglone and ethyl-gallate showed positive results in the blood–brain-barrier-specific PAMPA-BBB study. Juglone also possesses logPe values which indicates that it may be able to cross both the GI and BBB membranes via passive diffusion.
Conclusion: In our work, we provided new data on the chemical and biological characteristics of specialized plant metabolites of J. nigra, which may lay the foundation for the use of the plant as the source of drug candidate compounds.

University

Semmelweis University

Supervisor

Dr. Alberti-Dér Ágnes

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

phd.section.field

in doctoral studies before complex exam (PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

9007

Start

16:48

End

16:54