PhD Scientific Days 2025

Poster Session II. - B: Molecular Medicine

EV pretreatment enhances melanoma cell migration despite dual MAPK pathway inhibition

Előadó neve

Ms. Németh Afrodité

Neptun code

B4Y9EK

Előadó munkahelye

Pázmány Péter Catholic University

Előadó telefonszáma

+36202800061

Előadó e-mail címe

nemeth.afrodite@itk.ppke.hu

Az előadás címe

EV pretreatment enhances melanoma cell migration despite dual MAPK pathway inhibition

Szerző(k) neve és munkahelye

Afrodité Németh¹, Alexandra Simon¹, Gréta Lilla Bányai¹, Tamás Márton Garay²

1: Pázmány Péter Catholic University
2: Pázmány Péter Catholic University, Semmelweis University

Bemutatás módja

Poszter

Szekció

Poster Session II. - B: Molecular Medicine

Language of the presentation

English

Preferred session

Molecular Medicine

Összefoglaló szövege

Introduction:
Extracellular vesicles (EVs) are lipid bilayer-enclosed nanoparticles released by all cell types, playing key roles in intercellular communication and cancer progression. In previous work, we found that EVs promote melanoma cell migration even under BRAF inhibitor (BRAFi) treatment. However, this effect was diminished when MEK inhibitors (MEKi) were combined with BRAFi, possibly because those cell lines were not exposed to MEKi previously. To address this, we developed a melanoma cell line resistant to both encorafenib (BRAFi) and binimetinib (MEKi) to study EVs involvement in resistance.
Methods:
Resistant clones were generated by long-term low-dose drug exposure and validated by SRB assays. Morphology and behavior were assessed via videomicroscopy, proliferation via cell counting, and migration through semi-automated tracking with CellTracker. EVs were isolated from both sensitive and resistant cells and characterized by Qubit (protein), SPV (lipid), and NTA (particle content and size). EVs effects were tested using two strategies: (1) co-treatment with drugs and EVs, and (2) EV pretreatment 24 hours prior to drug exposure.
Results:
Resistant cells showed altered morphology resembling epithelial-to-mesenchymal transition (EMT), with elongation and decreased circularity. Their proliferation and migration were reduced, while the produced EVs’ lipid content was significantly elevated. EVs treatment did not increased cell proliferation under BRAFi-MEKi treatment, however, EV pretreatment enhanced migration under dual drug pressure. Simultaneous EV-drug treatment failed to show such effect.
Conclusion:
These results suggest that EVs contribute to therapy resistance not only through binding or encapsulating the drug but also by transferring complex signals that modulate cell behavior over time. The enhanced migration following EV pretreatment implies a potential role in metastasis even under targeted therapy.

University

Other, please specify in the next box

Other university, not listed above

Pázmány Péter Catholic University

Supervisor

Dr. Tamás Márton Garay

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

phd.section.field

in doctoral studies after complex exam (PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

9045

Start

19:12

End

19:18