PhD Scientific Days 2025

Poster Session III. - K: Theoretical and Translational Medicine

Modulated Electro-Hyperthermia Effects on mTOR-Related Gene Expression in TNBC

Előadó neve

Dániel Bócsi

Neptun code

EB66BF

Előadó munkahelye

Semmelweis, Institue of Translational Medicine

Előadó telefonszáma

+36302661131

Előadó e-mail címe

bocsidani@gmail.com

Az előadás címe

Modulated Electro-Hyperthermia Effects on mTOR-Related Gene Expression in TNBC

Szerző(k) neve és munkahelye

Dániel Bócsi¹

1: Semmelweis, Institue of Translational Medicine

Bemutatás módja

Poszter

Szekció

Poster Session III. - K: Theoretical and Translational Medicine

Language of the presentation

English

Preferred session

Theoretical and Translational Medicine

Összefoglaló szövege

Introduction: Triple-negative breast cancer (TNBC) is an aggressive subtype characterized by the absence of estrogen receptor, progesterone receptor, and HER2 expression. Modulated electro-hyperthermia (mEHT) has recently emerged as a promising noninvasive approach, offering a novel solution to the challenge of limited targeted therapy options for TNBC, exploiting the bioelectrical and metabolic differences between malignant and normal cells. In TNBC, the PI3K/AKT/mTOR signaling cascade is a critical driver of tumor progression, cell proliferation, and survival. Our study focuses on the interplay between mEHT and mTOR-related gene expression, particularly pathways involved in the metabolic switch.
Aims: Evaluate the combined effect of mEHT and pharmacological inhibition of PI3K or mTOR on metabolic gene expression in TNBC cell lines. Through this, we aim to clarify the potential of combined treatments to interfere with the mTOR-driven metabolic switch, and to map the mTOR-related gene expression changes that occur during metabolic changes during Thermo therapy.
Methods: Established murine TNBC cell lines (4T1) were used as an in vitro model. Cells were divided into three main experimental groups: (i) a sham control receiving no hyperthermic treatment or inhibitors; (ii) groups treated with either mEHT alone or various inhibitors; and (iii) groups treated with mEHT in combination with inhibitors (GDC-0941 for PI3K and AZD8055 for mTOR). Treatments were performed under controlled conditions, maintaining tumor tissue temperatures at 42 °C using localized hyperthermia. Gene expression changes were quantified by qPCR.
Results: mEHT treatment alone led to alterations in the expression of metabolic and mTOR-related genes. When administered in combination with GDC-0941 and AZD8055, an encouraging trend was observed, influencing key metabolic regulators and stress response genes.
Conclusion: Our findings indicate that mEHT not only directly impacts TNBC cell survival through physical hyperthermia but also modulates critical mediators of the mTOR pathway and the metabolic switch. When combined with targeted inhibitors, mEHT appears to influence signaling cascades that regulate cell metabolism and growth, offering a promising avenue for clinical translation in TNBC treatment.
Funding: NKFIH-EKÖP-KDP (2024-2.1.1-EKÖP), NKFIH OTKA (OTKA_K 145998), SOTE SE250+

University

Semmelweis University

Supervisor

Dr. Hamar Péter

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

phd.section.field

in doctoral studies before complex exam (PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

9066

Start

14:24

End

14:30