PhD Scientific Days 2025

Poster Session I. - T: Cardiovascular Medicine and Research

Isolation and Quantification of Circulating Extracellular Vesicles in Myocardial Homeostasis, Strain, and Ischemia States

Előadó neve

Dr. Bernáth-Nagy Dominika

Neptun code

PWJ6KL

Előadó munkahelye

Semmelweis University

Előadó telefonszáma

+36203853668

Előadó e-mail címe

bernath.nagy.dominika@semmelweis.hu

Az előadás címe

Isolation and Quantification of Circulating Extracellular Vesicles in Myocardial Homeostasis, Strain, and Ischemia States

Szerző(k) neve és munkahelye

Dominika Bernáth-Nagy¹, Jona Benjamin Krohn², Gabriel Jakob Trauner³, Melek Sükran Kalinyaprak³, Florian Sicklinger², Niklas Hartmann³, Daniela Pimonov³, Pál Ábrahám⁴, Florian Leuschner², Constanze Schmidt³, Felix Wiedmann³, Maren Harden³, Norbert Frey², Evangelos Giannitsis³

1: Heart and Vascular Centre of Semmelweis University, Budapest, Hungary; Department of Cardiology, Angiology and Pneumology, University Hospital Heidelberg, Heidelberg, Germany
2: Department of Cardiology, Angiology and Pneumology, University Hospital Heidelberg, Heidelberg, Germany; German Centre for Cardiovascular Research (DZHK) Partner Site Heidelberg/Mannheim, University of Heidelberg, Heidelberg, Germany
3: Department of Cardiology, Angiology and Pneumology, University Hospital Heidelberg, Heidelberg, Germany
4: Heart and Vascular Centre of Semmelweis University, Budapest, Hungary

Bemutatás módja

Poszter

Szekció

Poster Session I. - T: Cardiovascular Medicine and Research

Language of the presentation

English

Preferred session

Cardiovascular Medicine and Research

Összefoglaló szövege

Extracellular vesicles (EV) are membranous nanoparticles that are key elements of intercellular communication. EVs are released by a multitude of cell types and bear the potential to provide information on their cell of origin and their state. It has been shown that in vitro hypoxic conditioning triggers EV release by intact cardiomyocytes.
Translation of these in vitro findings to disease pathophysiologies in acute clinical settings is challenging. The aim of this study is the quantification of circulating EVs in different cardiovascular diseases (CVD) in patients, small- and large animal models.
Patients presenting to the Chest Pain Unit were recruited prospectively. Patients with non-ST elevation myocardial infarction (NSTEMI) (n=18) and with supraventricular tachyarrhythmia (SVT) (n=39) were identified and compared to a cohort of healthy volunteers (n=27). Peripheral blood was drawn, EVs were isolated and quantified with nanoparticle tracking analysis (NTA). The results of the NSTEMI patient cohort were validated in a murine model of minimal invasive myocardial infarction, whereas as a validation of the results of the SVT patient cohort a large animal model of induced SVT was utilized.
Quantification of circulating EVs in patients suffering NSTEMI revealed a ~3-fold increase (p<0.0001), and SVT-induced myocardial strain a ~2-fold increase (p<0.0001) in systemic EV release compared to control states. In the murine model of myocardial ischemia, NTA revealed a ~3-fold increase in total circulating EV release four hours after anterior wall infarction compared to sham controls bordering statistical significance (p=0.07). In the swine model of induced SVT, EVs isolated from plasma samples of specimen suffering from sustained atrial fibrillation showed a ~3-fold increase compared to control states (p<0.001).
In this extensive study on systemic EV release in patients as well as in small and large animal models of myocardial ischemia and strain, a quantitative increase in circulating EV release is demonstrated. These findings add to a growing body of evidence underlining the diagnostic value of circulatory EVs in CVDs and particularly its potential as an early-stage marker of myocardial strain or injury.
This study was funded by the University Hospital Heidelberg and Roche Diagnostics through an Investigator Initiated Trial contract.

University

Semmelweis University

Supervisor

Pál Ábrahám; Evangelos Giannitsis

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

phd.section.field

in doctoral studies before complex exam (PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

9064

Start

17:06

End

17:12