PhD Scientific Days 2025
Budapest, 7-9 July 2025
Poster Session I. - Q: Neurosciences
Simultaneous Administration of Phenylephrine with Pregabalin in Neuropathic Rats Produces an Acute Antiallodynic Effect
Előadó neve
Abbood Sarah, PhD
Neptun code
ANGGZ7
Előadó munkahelye
Semmelweis university
Előadó telefonszáma
+36204008873
Előadó e-mail címe
abbood.sarah@phd.semmelweis.hu
Az előadás címe
Simultaneous Administration of Phenylephrine with Pregabalin in Neuropathic Rats Produces an Acute Antiallodynic Effect
Szerző(k) neve és munkahelye
Sarah Abbood1,2, Nariman Essmat1,2, Imre Boldizsár1,2, Ildikó Miklya1,2, Kornél Király1,2, Laszlo G Harsing Jr1,2, Zoltan Giricz1,2, Csenger Kovácsházi1,2, Mahmoud Al-Khrasani1,2
1: Department of Pharmacology and Pharmacotherapy, Semmelweis University
2: Center for Pharmacology and Drug Research & Development, Semmelweis University, Budapest, Hungary
Bemutatás módja
Poszter
Szekció
Poster Session I. - Q: Neurosciences
Language of the presentation
English
Preferred session
Neurosciences
Összefoglaló szövege
Introduction: Neuropathic pain (NP) treatment remains unresolved thus far. The spinal cord is a crucial relay point in pain transmission and modulation. The adrenergic system modulates spinal pain perception, positioning it as a potential target for drug development. Phenylephrine (PE) is regarded as a selective α1-adrenoreceptor (AR) agonist, but previous studies, including ours, suggest it induces an indirect, non-calcium-dependent release of cytosolic norepinephrine (NE), similar to tricyclic antidepressants that enhance NE in the descending pain pathway, leading us to hypothesize that PE may have an antiallodynic effect.
Aims: Investigation of the effects of PE on mononeuropathic pain induced by partial sciatic nerve ligation (pSNL) in rats following per se intrathecal (it.) or oral administration, as well as in combination with pregabalin (PGB) after acute and chronic administrations. Elucidate the mechanisms of action and assess side effects related to motor function and blood pressure (BP).
Methods: Male Wistar rats underwent pSNL to induce tactile allodynia, the hallmark of NP. Allodynia was characterized by a decrease in the paw withdrawal threshold, measured by a Dynamic Plantar Aesthesiometer 2 weeks post-surgery. It. administration was achieved through direct injection, and oral administration was conducted by gavage. Prazosin and idazoxan are selective antagonists for α1 and α2 ARs, respectively, and were used to elucidate the mechanism of action of PE. The rotarod test was used to assess motor function. BP and heart rate were also assessed.
Results: After it, PE at 30 nmol /rat produced prazosin and idazoxan-sensitive antiallodynic effect in the operated paws. On the other hand, oral PE/PGB (5/25mg/kg) combination resulted in a significant antiallodynic effect following both acute and chronic treatment. This combination was devoid of side effects related to motor function or cardiovascular effects.
Conclusions: PE is a promising candidate for treating mononeuropathic pain when administered alone or in combination with PGB. This effect is mediated by α1 and α2 ARs; however, future studies are required to elucidate this issue. This work provides evidence of cooperation between ARs and voltage-gated calcium channels hosting α2δ subunits in the context of antiallodynia.
Funding: TKP 2021 EGA-25, SE 250+ Excellence PhD Scholarship, EKÖP 2024.
University
Semmelweis University
Supervisor
Mahmoud Al-Khrasani
Publication of my abstract
I give consent to the publication of my abstract on the website of the congress.
phd.section.field
in doctoral studies after complex exam (PhD)
Kind
Szabad
Status
elfogadva
Accepted presentation method
poszter
Előadás fájl jóváhagyás
nem rendelkezett róla
Előadó
8085
Start
17:30
End
17:36