PhD Scientific Days 2025

Poster Session II. - E: Pathological and Oncological Sciences

Comparison of Two Different PCR-based Methods for the Detection of Helicobacter Pylori and its Susceptibility to Clarithromycin in Complicated Peptic Ulcer Cases

Előadó neve

Biró Adrienn

Neptun code

T0PA29

Előadó munkahelye

Department of Pathology, Forensic and Insurance Medicine, Semmelweis University, Budapest, Hungary

Előadó telefonszáma

+36-30/486-8448

Előadó e-mail címe

biro.adrienn@phd.semmelweis.hu

Az előadás címe

Comparison of Two Different PCR-based Methods for the Detection of Helicobacter Pylori and its Susceptibility to Clarithromycin in Complicated Peptic Ulcer Cases

Szerző(k) neve és munkahelye

Adrienn Biró¹, Evelin Barna¹, Ildikó Szirtes¹, Anita Kurucz¹, Ákos Jakab¹, Erzsébet Rásó¹, Veronika Papp², Tamás Vass², Attila Szijártó², András Kiss¹, Éva Kocsmár¹, Tamás Barbai¹, Gábor Lotz¹

1: Department of Pathology, Forensic and Insurance Medicine, Semmelweis University, Budapest, Hungary
2: Department of Surgery, Transplantation and Gastroenterology, Semmelweis University, Budapest, Hungary

Bemutatás módja

Poszter

Szekció

Poster Session II. - E: Pathological and Oncological Sciences

Language of the presentation

English

Preferred session

Pathological and Oncological Sciences

Összefoglaló szövege

Introduction: Our aim was to compare two different PCR-based methods for detecting Helicobacter pylori (HP) and its susceptibility/resistance to clarithromycin (ClaSusc/ClaRes) in complicated (e.g. bleeding, perforated) peptic ulcer cases, where rapid eradication treatment following diagnosis, is important to reduce the risk of recurrent disease.
Methods: PCR was performed on paraffin-embedded, formalin-fixed gastroduodenal biopsy specimens from 102 patients who underwent emergency intervention and sampling for gastrointestinal bleeding and/or perforated peptic ulcer, using two different methods targeting the HP 23S rRNA gene. 1) With the HPS1/HPA1 primer pair, samples revealing a specific product by conventional PCR were further analysed by bidirectional Sanger sequencing. 2) The HPJ primer pair was used in FRET-based qPCR reaction, where HP and its ClaSusc/ClaRes status was detected by melting curve.
Results: Of the 102 patients, conventional PCR found 45 HP-negative cases (of which 2 were HP positive/ClaSusc by qPCR) and 57 HP-positive cases (52 ClaSusc and 5 ClaRes sequencing results). Detection by FRET-qPCR resulted in 53 HP-negative (of which 9 were HP-positive/ClaSusc and 1 HP-positive/ClaRes by conventional PCR/Sanger sequencing) and 49 HP-positive cases (with 32 ClaSusc and 17 ClaRes melting curve results).
Conclusion: Our data suggest that a significant proportion of complicated (bleeding/perforated) peptic ulcers are Helicobacter pylori associated. FRET-based qPCR is an ideal method for rapid, one-step identification of HP infection and its resistance to clarithromycin. However, further investigation of the reasons for differences in HP detection sensitivity and clarithromycin resistance rates detected by the two different PCR-based methods is needed to select the best approach.
Funding: K_22 142604, EKÖP-2024-72, 2023-2.1.2-KDP-2023-00016
Project no. 2023-2.1.2-KDP-2023-00016 has been implemented with the support provided by the Ministry of Culture and Innovation of Hungary from the National Research, Development and Innovation Fund, financed under the KDP-2023 funding scheme.

University

Semmelweis University

Supervisor

Prof. Dr. Lotz Gábor

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

phd.section.field

in doctoral studies before complex exam (PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

8014

Start

18:30

End

18:36