PhD Scientific Days 2025

Poster Session I. - Y: Surgical Medicine

Multi-scale Mechanics of Cryopreserved Human Arterial Allografts Across a One-year Period

Előadó neve

Dr. Kovács Gergely Imre

Neptun code

QUR8ST

Előadó munkahelye

Semmelweis University Dpt. of Vascular and Endovascular Surgery

Előadó telefonszáma

+36205172442

Előadó e-mail címe

k.gergo9@gmail.com

Az előadás címe

Multi-scale Mechanics of Cryopreserved Human Arterial Allografts Across a One-year Period

Szerző(k) neve és munkahelye

Gergely Imre Kovács MD¹, Dóra Haluszka PhD², Evelin Forró PhD², László Hidi M.D. PhD³, Prof. Péter Sótonyi M.D. PhD³

1: Semmelweis University Dpt. of Vascular and Endovascular Surgery
2: Dpt. of Biophysics and Radiation Biology, Semmelweis University
3: Dpt. of Vascular and Endovascular Surgery, Semmelweis University

Bemutatás módja

Poszter

Szekció

Poster Session I. - Y: Surgical Medicine

Language of the presentation

English

Preferred session

Surgical Medicine

Összefoglaló szövege

Introduction:
An operation under septic conditions presents significant challenges in vascular surgery: graft infection is a serious risk after synthetic vascular graft implantation. In these complicated cases, an infection-resistant alternative is essential to minimize the chance of reinfection.
Cryopreserved vascular allografts provide a suitable alternative for replacing an infected synthetic graft. Unfortunately, there are no international guidelines for the preparation, storage, or thawing of these vessel grafts.
Based on our decades of clinical experience with allograft use, we have observed a significant increase in the postoperative complication rates when allografts were implanted after more than 6 months of cryogenic storage.

Aims:
We aimed to compare the mechanical properties of cryopreserved arterial allografts over a one-year storage period.

Method:
Human arterial allograft samples explanted during multiorgan donations were frozen in a slow, controlled process using dimethyl sulfoxide. Samples were stored at -80 ˚C. Arteries were examined before cryopreservation (BC), immediately after cryopreservation (C0), and after one year of storage (C52). The extensibility and tensile strength properties were evaluated by uniaxial ring tests, and the elastic modulus was characterized by nanoindentation tests.
The results were analysed by Bland-Altman plots.

Results:
The extensibility between the BC-C0 samples was not remarkable (0.0143 ± 0.29), nor when comparing BC-C52 (0.0957 ± 0.58). The differences between tensile strength values were negligible between fresh and cryopreserved samples: BC-C0 (-0.0325 ± 0.0602 N/mm3) and BC-C52 (-0.0531 ± 0.11 N/mm3).
Regarding the Young’s modulus of cryopreserved arteries, our results showed no difference during the investigation period: BC-C0 (-0.1288 ± 3.14 GPa) and BC-C52 (-0.165 ± 2.80 GPa).

Conclusion:
We found no changes in the mechanical properties during the examination period. Our results indicate that the cryopreserved vascular allografts maintain mechanical stability for up to one year of storage.
The reduced graft patency beyond 6 months of storage is not related to the biomechanical capabilities of the grafts, therefore, further investigation is required to investigate the thrombogenicity, immunogenicity, and biocompatibility of these allografts.

Funding: This research received no funding.

University

Semmelweis University

Supervisor

Prof. Peter Sótonyi M.D.

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

phd.section.field

in doctoral studies before complex exam (PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

5713

Start

16:42

End

16:48