PhD Scientific Days 2025

Poster Session II. - J: Theoretical and Translational Medicine

The Role of HCN channels in the vascular adaptation to PCOS

Előadó neve

Dr. Mr. Bányai Bálint

Neptun code

OMZNHY

Előadó munkahelye

Semmelweis University department of physiology

Előadó telefonszáma

06705783752

Előadó e-mail címe

banyai.balint@gmail.com

Az előadás címe

The Role of HCN channels in the vascular adaptation to PCOS

Szerző(k) neve és munkahelye

Bálint Bányai¹, Mária Szekeres², Ágnes Pakuts¹, Stella Kiss¹, Skyler Senuma Pang¹, Szabolcs Várbíró³, Rita Benkő¹, Eszter M. Horváth¹

1: Semmelweis University, Department of Physiology
2: Semmelweis University Faculty of Helath Sciences Department of Morphology and Physiology
3: University of Szeged Department of Obstetrics and Gyneacology

Bemutatás módja

Poszter

Szekció

Poster Session II. - J: Theoretical and Translational Medicine

Language of the presentation

Hungarian

Preferred session

Theoretical and Translational Medicine

Összefoglaló szövege

Polycystic ovary syndrome (PCOS) is a common endocrine disorder among women of reproductive age, The main symptom is hyperandrogenism, often associated with Vitamin D deficiency in approximately 80% of cases. Additionally, PCOS is linked to an increased cardiovascular risk. Previous studies have identified the presence of all four isotypes of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels in the thoracic aorta of rats, with ivabradine, a non-selective HCN inhibitor, exhibiting vasorelaxation effects. Altered HCN expression has also been observed in diabetes mellitus.
This study aimed to investigate changes in HCN-dependent vascular responses in a rat model of hyperandrogenism and evaluate the potential impact of Vitamin D supplementation.
A total of 44 female Wistar rats (weighing 70–90 g) were assigned to four experimental groups: control (C), Vitamin D-supplemented (D, 140 IU/100 g body weight), testosterone-treated (T, daily 3.3 mg/100 g transdermal Androgel), and a combination group receiving both Vitamin D and testosterone (T+D). After eight weeks, thoracic aortic segments were isolated to wire myography to assess ivabradine-induced relaxation following phenylephrine pre-contraction, and the remaining vessel segments after formalin fixed paraffin embedding going under immunohistochemical analysis against HCN1-4 .
Results demonstrated that Vitamin D supplementation marginally enhanced ivabradine-induced vasorelaxation (p=0,062). HCN1 expression was significantly elevated in the combined T+D group compared to the other three groups. HCN2 expression was elevated in testosterone-treated rats but this effect was mitigated by Vitamin D supplementation. HCN3 expression was significantly reduced in both Vitamin D-treated groups, while HCN4 was upregulated in testosterone-treated groups compared to the controls.
These findings suggest that HCN channels may play a role in the vascular alterations observed in PCOS and indicate a potential cardiovascular benefit of ivabradine in this condition.

Funded by the Department of Physhiology Semmelweis University

University

Semmelweis University

Supervisor

Eszter M. Horváth

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

phd.section.field

after finishing doctoral studies with absolutorium (PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

6054

Start

18:12

End

18:18