PhD Scientific Days 2025
Budapest, 7-9 July 2025
Poster Session III. - R: Neurosciences
Changes in Neuronal Network and Glial Activation in Human Organotypic Brain Slice Cultures
Előadó neve
Stelcz Rebeka, MSc
Neptun code
SA70CG
Előadó munkahelye
HUN-REN Research Centre for Natural Sciences
Előadó telefonszáma
+36308522150
Előadó e-mail címe
stelcz.rebeka@phd.semmelweis.hu
Az előadás címe
Changes in Neuronal Network and Glial Activation in Human Organotypic Brain Slice Cultures
Szerző(k) neve és munkahelye
Rebeka Stelcz1,2, Nour Essam Fawzy Ahmed Aly1,3, Réka Bod1,2, Eszter Juhász1, Kinga Tóth1, Estilla Zsófia Tóth4, István Ulbert1,3,5, Lucia Wittner1,2,5
1: HUN-REN Research Centre for Natural Sciences
2: Semmelweis University, János Szentágothai Neurosciences Division
3: PPCU, Roska Tamás Doctoral School of Sciences and Technology
4: Hun-Ren Research Centre for Natural Sciences
5: Semmelweis University, Department of Neurosurgery
Bemutatás módja
Poszter
Szekció
Poster Session III. - R: Neurosciences
Language of the presentation
English
Preferred session
Neurosciences
Összefoglaló szövege
Introduction: Several animal and computational models help study the central nervous system's properties, advancing our understanding of neuronal behaviour, neurodegenerative diseases, and potential treatments. Despite their value, translating findings from animal models to humans is limited by the human brain's unique cellular, molecular, and network features. Human organotypic brain tissue cultures offer an excellent opportunity to explore the characteristics of the human brain on the cellular and molecular level and assess novel pharmaceutical compounds in a more realistic environment.
Aims: In this study, we explored neuronal degeneration and loss, as well as the degree of glial activity along the culture period.
Method: Postoperative brain tissues were sliced and maintained in six-well plates under sterile conditions. Two culture media (artificial culturing medium, human cerebrospinal fluid) and distinct antibiotics, antimycotics (penicillin, streptomycin, amphotericin) for infection prevention were tested. Morphological modifications of neurons were investigated using immunostaining against the neuronal marker NeuN and the dendrite marker MAP2. Changes in astrocyte and microglial activation patterns were detected with anti-GFAP and anti-IBA1 staining, respectively. These methods enabled us to assess the extent to which the cytoarchitecture of the neocortical tissue culture is preserved in comparison to its acute state regarding the neuronal cell density, morphology and glial activation.
Results: We found that while organotypic cultures maintain overall tissue architecture, considerable changes occur over time. Neuronal density was decreasing mostly in the early culturing period, while astroglial and microglial activation was seen at the longer term during the culturing period.
Conclusion: According to the results, although human organotypic cultures preserve their structure, they show remarkable reorganization with time, highlighting the importance of continous anatomical examinations of the cultures to better understand the morphological modifications and their relation to the physiology of these slice cultures.
Funding:
National Research, Development and Innovation Office K137886 and Bolyai Fellowship
Hungarian Brain Research Program NAP2022-I-8/2022,
PharmaLab RRF-2.3.1-21-2022-00015
NKFIH Advanced 150799, OTKA K137886
University
Semmelweis University
Supervisor
Lucia Wittner, DSc
Publication of my abstract
I give consent to the publication of my abstract on the website of the congress.
Kind
Szabad
Status
elfogadva
Accepted presentation method
poszter
Előadás fájl jóváhagyás
nem rendelkezett róla
Előadó
9141
Start
14:54
End
15:00