PhD Scientific Days 2025

Poster Session I. - F: Pharmaceutical Sciences and Health Technologies

Formulation development of an orally administered liposomal delivery system

Előadó neve

Ms. Molnár Petra

Neptun code

VJ5144

Előadó munkahelye

Semmelweis University Department of Pharmaceutics

Előadó telefonszáma

+36203106650

Előadó e-mail címe

petraa.molnar@gmail.com

Az előadás címe

Formulation development of an orally administered liposomal delivery system

Szerző(k) neve és munkahelye

Petra Molnár¹

1: Semmelweis University Department of Pharmaceutics

Bemutatás módja

Poszter

Szekció

Poster Session I. - F: Pharmaceutical Sciences and Health Technologies

Language of the presentation

Hungarian

Preferred session

Pharmaceutical Sciences and Health Technologies

Összefoglaló szövege

Introduction: Most liposomal drug delivery systems are designed for parenteral use; oral formulations are less common due to challenges in gastrointestinal stability and targeted absorption. The selection of the lipid compositions and stabilizing excipients is crucial in the design of oral liposomal formulations. Riboflavin (vitamin B2), a light-sensitive compound essential in cellular energy metabolism, exhibits synergistic effects with certain antibiotics. Liposomes may offer a promising strategy for preserving their stability and efficacy in the case of oral application.
Aims: We aimed to develop liposomes suitable for oral drug delivery. To increase the efficiency of formulation development, we optimized a thin-film hydration method that allows rapid sample preparation and reproducible production of multiple compositions simultaneously. Following compositional screening, our goal extended to the production and characterization of riboflavin-containing liposomes.
Methods: Lipid films were prepared using vacuum centrifugation at various temperatures to evaluate their impact on macroscopic film appearance and liposome size distribution. 9 lecithin-based liposomal compositions with varying ratios of cholesterol and bile salts were produced and compared. After hydration and extrudation, size distribution and zeta potential were measured by Zetasizer. B2 entrapment efficiency was determined by UV spectroscopy.
Results: We established a rapid lipid film formation method (50°C, 200 rpm) that allowed the preparation of homogeneous and stable liposome suspensions. Increasing bile salt content significantly decreased the average size of liposomes and increased zeta potential values (p<0.05); the cholesterol content had no significant effect within the study range. The size distribution of the different B2-loaded liposomes were homogeneous (PDI<0.1), with average sizes of 120–140 nm and the absolute value of zeta potential >30 mV. Their entrapment efficiency was below 50%; however, using antioxidant excipients is applicable as an improvement materials.
Conclusion: The optimized method supports efficient lipid film preparation, facilitating research requiring high sample throughput. Liposomes may protect the active ingredient against gastrointestinal conditions during oral administration, providing enhanced stability and therapeutic effectiveness.
Funding: EKÖP-2024.

University

Semmelweis University

Supervisor

Dr Andrea Kovács, Dr. Nóra Mike-Kaszás

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

phd.section.field

before finishing undergraduate studies (TDK, MD-PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

9153

Start

16:42

End

16:48