PhD Scientific Days 2025

Poster Session I. - Q: Neurosciences

Induced Müller Glia Proliferation in the Adult Human Retina

Előadó neve

Dr. Gerendas Lili

Neptun code

B7HMV4

Előadó munkahelye

Department of Anatomy, Histology and Embryology, Semmelweis University

Előadó telefonszáma

+36302404680

Előadó e-mail címe

gerendaslili@semmelweis.hu

Az előadás címe

Induced Müller Glia Proliferation in the Adult Human Retina

Szerző(k) neve és munkahelye

Lili Gerendas¹, Magda Daniel¹, Teadora Tyler¹, Ferenc Kilin¹, Arnold Szabo¹

1: Department of Anatomy, Histology and Embryology, Semmelweis University

Bemutatás módja

Poszter

Szekció

Poster Session I. - Q: Neurosciences

Language of the presentation

English

Preferred session

Neurosciences

Összefoglaló szövege

Aims: Regenerative capacity in the mature human retina remains largely unexplored. Unlike mammals, certain vertebrates regenerate retinal neurons through Müller glia, which re-enter the cell cycle and transform into multipotent progenitor cells capable of generating glial and neuronal lineages. This study explores the mitotic potential of Müller glia using long-term organotypic cultures of postmortem adult human retina.
Methods: Retinal explants, including central and peripheral regions, were obtained from over 30 multi-organ donors aged 18 to 68, encompassing males and females. The explants were cultured under defined conditions for 70 days, with EdU or BrdU as proliferation markers, and exposed to different concentrations of the inducing agents Wnt-3a, BIO, MSG, and FGF-2. The cultures were analyzed using immunohistochemistry, flow cytometry, and single-cell RNA sequencing.
Results: Single-cell RNA sequencing revealed that the transcriptomic profiles of the cultured cells closely resemble those of fresh retinal tissue. All the inducing agents tested effectively promoted cell proliferation in both peripheral and central regions, with FGF-2 emerging as the most potent inducer. Immunohistochemistry revealed that over 70% of the Edu-positive postmitotic cells were Müller glia. Flow cytometry confirmed a significantly higher number of EdU+ cells in the treated groups than controls, indicating robust cell proliferation.
The density of dividing cells was consistent from the ora serrata to the mid-periphery. Independently of age and sex, all donors exhibited high levels of induced cell division, demonstrating that Müller cells retain their ability to divide beyond youth.
Conclusion: This study demonstrates the mitotic potential of Müller glia from mature human retinas, including those of older donors, and enhances our understanding of human retinal regenerative biology.
Funding: Élvonal 138726

University

Semmelweis University

Supervisor

Arnold Szabo

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

phd.section.field

in doctoral studies after complex exam (PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

9183

Start

17:06

End

17:12