PhD Scientific Days 2025

Poster Session III. - C: Molecular Medicine

A simple and rapid protocol to isolate Outer Membrane Vesicles from E. coli

Előadó neve

Seregélyes Gábor

Előadó munkahelye

Dept. of Genetics, Cell- and Immunobiology, Semmelweis University

Előadó telefonszáma

+36 1 210 2930 / 56469

Előadó e-mail címe

ricosereg@gmail.com

Az előadás címe

A simple and rapid protocol to isolate Outer Membrane Vesicles from E. coli

Szerző(k) neve és munkahelye

G. Seregélyes¹, O. Dobay², E.I. Buzás^1,3, É. Pállinger¹

1: Dept. of Genetics, Cell- and Immunobiology, Semmelweis University
2: Inst. of Medical Microbiology, Semmelweis University, Faculty of Medicine
3: HUN-REN-SU, Budapest, Hungary

Bemutatás módja

Poszter

Szekció

Poster Session III. - C: Molecular Medicine

Language of the presentation

English

Összefoglaló szövege

The production of extracellular vesicles is a common feature of prokaryotic cells. Gramnegative bacteria typically release 20-250 nm outer membrane vesicles (OMVs) during each life cycle, but environmental factors have a major impact on their molecular cargo. The
membrane composition of OMVs is similar to the outer membrane of bacteria, including LPS and specific proteins. The cargo contains mainly periplasmic proteins and proteoglycans in the resting state, whereas cytoplasmic proteins and nucleic acids appear in stress-induced OMVs.
The biological function of OMVs is quite diverse. They are suitable for transporting various biomolecules to other bacteria and are able to help bacterial survival (horizontal gene transfer) and delivering toxins into eukaryotic cells thereby causing infection.
In clinical practice, OMVs have diagnostic and therapeutic potential, including identification of infectious agents (e.g. sepsis) and vaccination. Therefore, it is very important to characterize the OMV-mediated functions in vitro.
The aim of our study was to standardize a simple, rapid and reproducible method for isolating OMVs that can be used for in vitro laboratory experiments to investigate OMVmediated effects.
The Gram-negative E.coli strain was used for the experiments. According to MISEV 2023 NTA, FACS, BCA and lipid assay were performed to characterize the bacterial OMVs

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

7329

Start

15:18

End

15:24