PhD Scientific Days 2026

Pharmaceutical Sciences and Health Technologies 1.

Phytochemical Investigation of Rosmarinic Acid Derivatives and Their Conversion Products from Nepetoideae Species

Előadó neve

Ms. Nazli, Adila

Neptune code

U27GVR

Előadó munkahelye

Department of Pharmacognosy, Semmelweis University, Üllői út 26, 1085 Budapest, Hungary

Előadó telefonszáma

+36704143381

Előadó e-mail címe

adilanazli44@gmail.com

Az előadás címe

Phytochemical Investigation of Rosmarinic Acid Derivatives and Their Conversion Products from Nepetoideae Species

Szerző(k) neve és munkahelye

Adila Nazli¹, Bernadett Szögi-Tatár¹, Gergő Tóth², Szilvia Bősze³, Szabolcs Béni⁴, Imre Boldizsár⁵

1: Department of Pharmacognosy, Semmelweis University, Üllői út 26, 1085 Budapest, Hungary; Center for Pharmacology and Drug Research & Development, Semmelweis University, Üllői út 26, 1085 Budapest, Hungary
2: Department of Pharmaceutical Chemistry, Semmelweis University, Hőgyes Endre u. 9, 1092 Budapest, Hungary; Center for Pharmacology and Drug Research & Development, Semmelweis University, Üllői út 26, 1085 Budapest, Hungary
3: HUN-REN-ELTE Research Group of Peptide Chemistry, Hungarian Research Network, ELTE Eötvös Loránd University, Pázmány Péter sétány 1/A, 1117 Budapest, Hungary; Department of Genetics, Cell- and Immunobiology, Semmelweis University, Nagyvárad tér 4, 1089 Budapest, Hungary
4: Integrative Health and Environmental Analysis Research Laboratory, Department of Analytical Chemistry, Institute of Chemistry, Eötvös Loránd University, Pázmány Péter sétány 1/a, 1117 Budapest, Hungary
5: Department of Pharmacognosy, Semmelweis University, Üllői út 26, 1085 Budapest, Hungary; Center for Pharmacology and Drug Research & Development, Semmelweis University, Üllői út 26, 1085 Budapest, Hungary; Department of Plant Anatomy, Institute of Biology, Eötvös Loránd University, Pázmány Péter sétány 1/C, 1117 Budapest, Hungary

Bemutatás módja

Szóbeli

Szekció

Pharmaceutical Sciences and Health Technologies 1.

Language of the presentation

English

Preferred session

Pharmaceutical Sciences and Health Technologies

Összefoglaló szövege

Introduction: Rosmarinic acid and its related compounds are mainly found in plants belonging to the Nepetoideae subfamily of the Lamiaceae family. Salvia officinalis (sage) is a well-known culinary and medicinal herb, widely recognized as a main source of rosmarinic acid. Salvia verticillata (lilac sage), Salvia pratensis (meadow sage), Salvia nemorosa (woodland sage), Salvia sclarea (clary sage), and Glechoma hirsuta (creeping charley) are closely related species within the subfamily Nepetoideae. Their phytochemical composition remains insufficiently characterized, with existing studies focusing primarily on the aerial parts.
Aims and Methods: Current study was designed to 1) depict the accumulation of the compounds in various organs of selected Nepetoideae species by HPLC-HR-MS/MS, 2) analyze changes in the metabolite composition during endogenous enzyme treatment of the extracts, 3) define optimum tissues and enzyme treatment conditions, allowing for the isolation of compounds, 4) determine the structures of the isolated compounds by NMR, and 5) evaluate the antioxidant and anti-proliferative potential of isolated compounds.
Results: Without enzyme treatment, we could isolate; i) rosmarinic acid-3-O-β-D-glucoside (1), rosmarinic acid (3), and a novel related compound (4), from the roots of S. verticillata; ii) salvianolic acids (2 and 5) from the roots and rhizomes of S. pratensis and G. hirsuta, respectively. As a result of optimized endogenous enzyme treatment, conversion products were formed with the loss of a formyl moiety from all the parent compounds, leading to the isolation of nepetoidines (7 and 8) in significantly higher yields than those previously reported. Furthermore, a novel salvianolic acid derivative (6) could also be isolated. Isolated compounds showed structure-specific antioxidant and cytostatic activities.
Conclusion: Two novel salvianolic acid derivatives were reported, along with an alternative strategy to obtain nepetoidines in higher yields.
Funding: This project was supported by the 2025-2.1.1-EKÖP-2025-00014 University Researchers’ Scholarship Program of the Ministry for Culture and Innovation from the source of the National Research, Development and Innovation Fund (EKÖP-2025-350) (A.N); the National Research, Development and Innovation Office, Hungary (grants: OTKA NKFIH K-142904 (S.B.), FK-146930 (G.T.).

University

Semmelweis University

Supervisor

Szabolcs Béni, Imre Boldizsár

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

phd.section.field

in doctoral studies after complex exam (PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

jóváhagyta

Előadó

7406

Start

14:45

End

14:55