PhD Scientific Days 2026

Poster Session 3.J - Theoretical and Translational Medicine

Preclinical comparison of modulated electro-hyperthermia impulse mode: Superior anti-tumor efficacy over continuous mode in a Triple-Negative Breast Cancer model

Előadó neve

Mr. Bócsi, Dániel

Neptune code

eb66bf

Előadó munkahelye

Semmelweis University, Institute of Clinical Pathophysiology

Előadó telefonszáma

06302661131

Előadó e-mail címe

bocsidani@gmail.com

Az előadás címe

Preclinical comparison of modulated electro-hyperthermia impulse mode: Superior anti-tumor efficacy over continuous mode in a Triple-Negative Breast Cancer model

Szerző(k) neve és munkahelye

Dániel Bócsi¹, Qusay Abdalla¹, Mohamed Elsalahaty¹, Hakim Bahlok¹, Eslam Abdalalem¹, Yibo Gu¹, Ebsa Ahmed¹, Mingming He¹, Csaba Schvarcz¹, Koós Zoltán¹, Cedrik Kintscher¹, Hamar Péter¹

1: Institute of Clinical Pathophysiology

Bemutatás módja

Poszter

Szekció

Poster Session 3.J - Theoretical and Translational Medicine

Language of the presentation

English

Preferred session

Theoretical and Translational Medicine

Összefoglaló szövege

Introduction:
Modulated electro-hyperthermia (mEHT) is a non-invasive adjuvant therapy applying 13.56 MHz radiofrequency to induce selective tumor damage. In current clinical practice, both continuous mode (CM) and the newer impulse mode (IM) devices are actively used; however, a direct side-by-side preclinical comparison of their efficacy and molecular impact remain limited. Our study aimed to provide a head-to-head comparison of CM and IM using sham-treated controls.
Materials and Methods:
Orthotopic 4T1 triple-negative breast cancer (TNBC) tumors were established in BALB/c mice. Animals were randomized to sham, continuous mode mEHT, or impulse mode mEHT groups. Treatments (30 min, 42.5°C) were delivered four times at 48-hour intervals. Tumor progression was monitored by ultrasound and caliper measurements. Final tumor weight was determined at sacrifice.
Results and Discussion:
Both treatment modalities significantly reduced tumor growth compared to Sham controls.
(Sham: 332.3±73.68 mg; CM: 213.2±73.68 mg; IM: 130±29.74 mg; IM vs. Sham: p = 0.0005; CM vs. Sham: p = 0.0342). Impulse mode demonstrated significantly greater efficacy than continuous mode (IM vs. CM: p = 0.0271), notably, ultrasound follow-up revealed that IM induced a significant reduction in tumor volume as early as after the second mEHT treatment (p = 0.0033), whereas CM did not. Stable body weights suggested good tolerability. Histopathological tumor destruction ratio (TDR) analysis further supported these findings, showing a significant increase in tumor destruction in CM compared with sham (p = 0.0203), with IM achieving a further significant improvement over CM (p = 0.0093). Comparative investigation of the treatment-induced molecular stress responses and apoptotic pathways is currently ongoing.
Conclusion:
Our study provides a controlled preclinical comparison of the two clinically used mEHT modalities, demonstrating that impulse mode offers superior tumor growth control over continuous mode in a TNBC model. These findings support further translational investigation of impulse mode and provide a rationale for future comparative clinical evaluation.
Funding: NKFIH-EKÖP-KDP (2024-2.1.2-EKÖP-KDP / EKÖP-KDP-2024-21), NKFIH OTKA (OTKA_K 145998), SOTE SE250+ (SE250_2026-9)

University

Semmelweis University

Supervisor

Dr. Hamar Péter

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

phd.section.field

in doctoral studies before complex exam (PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

9066

Start

13:42

End

13:45