PhD Scientific Days 2026

Poster Session 1.A - Molecular Medicine

Investigation of Extracellular Vesicle Corona Formation with Label-Free Optical Waveguide Lightmode Spectroscopy Method

Előadó neve

Mr. Bokrossy, Péter

Neptune code

G93AWK

Előadó munkahelye

Pediatric Center, MTA Center of Excellence, Semmelweis University; Budapest

Előadó telefonszáma

+36708667254

Előadó e-mail címe

bokrossy.p@gmail.com

Az előadás címe

Investigation of Extracellular Vesicle Corona Formation with Label-Free Optical Waveguide Lightmode Spectroscopy Method

Szerző(k) neve és munkahelye

Péter Bokrossy¹, Beáta Szebeni², Domonkos Pap², Apor Veres-Székely², Csenge Szász³, Jamila Raufi³, Tamás Visnovitz⁴, Lenzinger Dorina⁵, Éva Pállinger⁵, Nóra Fekete⁵, Zoltán Varga⁶, Judith Mihály⁷, Edit Buzás⁸, Attila J Szabó², Ádám Vannay², Nóra Adányi⁹

1: Pediatric Center, MTA Center of Excellence, Semmelweis University; Budapest
2: Pediatric Center, MTA Center of Excellence, Semmelweis University; Budapest, Hungary; HUN-REN–SU Pediatrics and Nephrology Research Group, Budapest, Hungary
3: Pediatric Center, MTA Center of Excellence, Semmelweis University; Budapest, Hungary
4: Institute of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary; HUN-REN SU, Translational Extracellular Vesicles Research Group, Budapest, Hungary
5: Institute of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary
6: Biological Nanochemistry Research Group, Institute of Materials and Environmental Chemistry, HUN-REN Research Centre for Natural Sciences, Budapest, Hungary; Department of Physical Chemistry and Materials Science, Budapest University of Technology and Economics, Budapest, Hungary; Semmelweis University, Faculty of Medicine, Department of Biophysics and Radiation Biology - HUN-REN Office for Supported Research Groups, Budapest, Hungary
7: Biological Nanochemistry Research Group, Institute of Materials and Environmental Chemistry, HUN-REN Research Centre for Natural Sciences, Budapest, Hungary
8: Institute of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary; HUN-REN SU, Translational Extracellular Vesicles Research Group, Budapest, Hungary; HCEMM-SU Extracellular Vesicles Research Group, Budapest, Hungary
9: Insuline BT. Budapest, Hungary

Bemutatás módja

Poszter

Szekció

Poster Session 1.A - Molecular Medicine

Language of the presentation

English

Preferred session

Molecular Medicine

Összefoglaló szövege

Introduction
Extracellular vesicles (EVs) are lipid bilayer-delimited particles that are naturally released from almost all types of cells into the extracellular environment. During their biogenesis and release to the extracellular space, EVs adsorb biomolecules, collectively designated as EV corona. Among corona components, proteins like albumin can be particularly important. In this study, we developed a novel method for immobilizing EVs and assessing their capacity to bind human serum albumin (HSA) as a model protein for corona formation.

Methods
The method development for EV-HSA binding was performed using optical waveguide lightmode spectroscopy (OWLS) flow-injection analyser (FIA) system. All experiments were conducted using tris buffer (42 mM, pH 7.4) as flow-through medium, under varying flow rates and flow cell temperatures. To evaluate EV immobilisation and surface passivation efficiency for HSA, chip surfaces were treated with poly-L-lysine (PLL) of varying molecular weights, pH, and concentrations. The optimal measuring range of protein concentration was determined using the previously optimised surface treatment and a range of HSA concentrations. Mathematical modelling was used to estimate the theoretical number of HSA can be bound on the EVs surface, then proof measurements were performed to support these calculations.
Results
To conclude the optimisation, the final parameters were found as 10 ng/mL PLL (15-30 kDa) in pH 7.4 buffer solution, at 25 °C; with 100 µl/min flow rate, the isolated EVs were diluted 4.5×1010 particle/mL and the optimal measuring range of HSA can be defined between 1-100 ng/mL. The proof measurements for the mathematical modelling revealed that the results corresponded in magnitude to the theoretical maximum HSA can be bound on the EVs surface, indicating the accuracy of the OWLS measurements.

Conclusions
Our OWLS method is applicable for the examination of real-time EV corona formation.

Funding
K-142728, K-131594, ADVANCED-150077, TKP2021-EGA-24, TKP2021-EGA-23, EKÖP-2025-367; EKÖP-2025-376; VEKOP-2.3.2-162016-00002, VEKOP-2.3.3-15-2017-00016, RRF-2.3.121-2022-00003; NKFIH 150767 Advanced

University

Semmelweis University

Supervisor

Dr. Ádám Vannay, Dr. Beáta Szebeni

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

phd.section.field

in doctoral studies after complex exam (PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

8110

Start

16:30

End

16:33