PhD Scientific Days 2026

Poster Session 2.B - Molecular Medicine

Generation and characterization of a novel primordial germ cell-specific monoclonal antibody

Előadó neve

Ms. Kaczur, Bettina, MSc

Neptune code

F1BYKO

Előadó munkahelye

Department of Anatomy, Histology and Embryology

Előadó telefonszáma

06202860692

Előadó e-mail címe

kaczur.bettina@phd.semmelweis.hu

Az előadás címe

Generation and characterization of a novel primordial germ cell-specific monoclonal antibody

Szerző(k) neve és munkahelye

Bettina Kaczur¹, Nóra Fejszák¹, Emőke Szőcs¹, Helga Hajnalka Farkas¹, Viktória Halasy¹, Nándor Nagy¹

1: Department of Anatomy, Histology and Embryology

Bemutatás módja

Poszter

Szekció

Poster Session 2.B - Molecular Medicine

Language of the presentation

English

Preferred session

Molecular Medicine

Összefoglaló szövege

Primordial germ cells (PGCs) are the stem cells of the germline, playing a crucial role in gametogenesis and the transmission of genetic information. Accurate identification and characterization of PGCs are critical for research in developmental biology, germline preservation, and reproductive science. This research project aims to characterize a novel mouse monoclonal antibody (clone name: 30B6) originally generated by our research group for the detection of B-cells. Preliminary immunocytochemical analyses revealed that, in addition to labelling early B-cells in the bursa of Fabricius of avian embryos, 30B6 also specifically recognizes PGCs. In avian species, reliable identification of PGCs requires multiple antibodies, as no single marker uniformly labels PGC populations across developmental stages. We hypothesize that 30B6 exhibits greater sensitivity and specificity for PGC detection than currently available markers and may have broader applicability across various species and developmental stages. We examined the binding characteristics and specificity of the antibody in different developmental stages of chicken embryos and their lymphoid organs using immunohistochemical and immunofluorescent staining techniques. Our findings indicate that the 30B6 antibody specifically labels PGCs in birds from early developmental stages, showing intense immunoreactivity in the germinal crescent of one-day-old embryos. 30B6 offers more distinct labelling of PGCs in the gonadal ridges compared to commercially available markers. Additionally, 30B6 recognizes goblet cells and their product lining the intestinal epithelium in birds (chicken, quail, partridge), mammals (human, not rodents), and reptiles, suggesting broader cross-species specificity. Furthermore, 30B6 stains the human primordial germ cells in an 8-week-old embryo. Taken together, these results demonstrate that the 30B6 monoclonal antibody is a novel and versatile tool that can enhance the accurate identification of avian and human PGCs and support further research in germline development.
Funding: Dr. Nándor Nagy OTKA grant (138664) and SE 250+ Excellence PhD Scholarship

University

Semmelweis University

Supervisor

Dr. Nándor Nagy

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

phd.section.field

in doctoral studies before complex exam (PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

9750

Start

18:12

End

18:15