PhD Scientific Days 2026

Poster Session 3.N - Neurosciences

Cortical and subcortical afferents of the thalamic nucleus reuniens

Előadó neve

Ms. Kóta, Kata

Neptune code

DKETYK

Előadó munkahelye

HUN-REN Institute of Experimental Medicine

Előadó telefonszáma

06307205528

Előadó e-mail címe

kotakata02@gmail.com

Az előadás címe

Cortical and subcortical afferents of the thalamic nucleus reuniens

Szerző(k) neve és munkahelye

Kata Kóta^1,2, Ágoston Keszei¹, László Acsády¹, László Biró¹

1: Lendület Laboratory of Thalamus Research, HUN-REN Institute of Experimental Medicine
2: János Szentágothai Doctoral School of Neurosciences, Semmelweis University

Bemutatás módja

Poszter

Szekció

Poster Session 3.N - Neurosciences

Language of the presentation

English

Preferred session

Neurosciences

Összefoglaló szövege

Introduction: The thalamic nucleus reuniens (Re) coordinates emotion-, memory-related behaviors and is involved in numerous pathological conditions (e.g Alzheimer’s disease). It orchestrates medial prefrontal–hippocampal communication and subcortical influences. However, the origin of subcortical excitatory (vGLUT2+) and inhibitory (vGAT+) inputs in Re is unknown. It is unclear how these inputs are organized within the Re and relative to cortical (vGLUT1+) inputs.
Aims: We aimed to identify the excitatory and inhibitory afferents of Re and examine the distribution of axonal labelling of cortical and subcortical inputs in the different subregions of Re and relative to each other.
Methods: We used retrograde and anterograde viral tract-tracing in transgenic mice lines followed by immunohistochemical labelling and analysis with confocal microscopy.
Results: We examined the density of presynaptic terminals by immunohistochemistry for vesicular glutamate transporter 1 and 2 (vGLUT1, vGLUT2), and the vesicular GABA transporter vGAT. vGLUT1-immunostaining was broadly uniform within the Re and was not different compared to that of the adjacent nuclei. In contrast, Re displayed stronger vGLUT2+ and vGAT+ terminal staining relative to its neighbors. Viral tracing revealed widespread and selective, subcortical vGAT+ and vGLUT2+ inputs mainly from the hypothalamic and brainstem regions, respectively. Most of these inputs were compartmentalized within the Re. Cortical inputs arising from different cortical territories also displayed highly heterogeneous innervation pattern with the Re.
Conclusion: These findings indicate that Re receives strong and selective subcortical GABAergic and glutamatergic inputs, but the different sets of cortical and subcortical inputs display robust compartmentalization resulting in a complex territorial mosaic specialized to integrate different afferents.
Funding: This project was supported by the 2025-2.1.1-eköp-2025-00014 University Research Scholarship Programme of the Ministry for Culture and Innovation from the Source of the National Research, Development and Innovation fund; by the János Bolyai Research Scholarship of the Hungarian Academy of Sciences; by the National Academy of Scientist Education Program of the National Biomedical Foundation underthe sponsorship of the Hungarian Ministry of Culture and Innovation and by NKFI (153367).

University

Semmelweis University

Supervisor

László Biró, László Acsády

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

phd.section.field

before finishing undergraduate studies (TDK, MD-PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

9727

Start

14:24

End

14:27