PhD Scientific Days 2026

Poster Session 2.B - Molecular Medicine

Antifibrotic Effects of Spirulina maxima Derived Extracellular Vesicles

Előadó neve

Raufi, Jamila, MSc

Neptune code

SGKAVL

Előadó munkahelye

Pediatric Center, MTA Center of Excellence, Semmelweis University, Budapest, Hungary

Előadó telefonszáma

+36703228299

Előadó e-mail címe

raufi.jamila@phd.semmelweis.hu

Az előadás címe

Antifibrotic Effects of Spirulina maxima Derived Extracellular Vesicles

Szerző(k) neve és munkahelye

Jamila Raufi¹, Beáta Szebeni^1,2, Mária Bernáth¹, Péter Bokrossy¹, Domonkos Pap^1,2, Zoltán Molnár³, Tamás Mészáros^4,5,6, Dorina Lenzinger⁷, Tamás Visnovitz^7,8, Marcell Pálmai⁹, Judith Mihály⁹, Zoltán Varga^9,10,11, Nóra Fekete⁷, Éva Pállinger⁷, Apor Veres-Székely^1,2, Csenge Szász¹, Edit Buzás^7,12, Attila J. Szabó^1,2, Ádám Vannay^1,2

1: Pediatric Center, MTA Center of Excellence, Semmelweis University, Budapest, Hungary
2: HUN-REN-SU Pediatrics and Nephrology Research Group, Budapest, Hungary
3: Department of Plant Sciences, Albert Kázmér Faculty of Agricultural and Food Sciences in Mosonmagyaróvár, Széchenyi István University, Mosonmagyaróvár, Hungary
4: Department of Translational Medicine, Nanomedicine Research and Education Center, Semmelweis University, Budapest, Hungary
5: SeroScience Ltd., Budapest, Hungary
6: Heart and Vascular Center, Faculty of Medicine, Semmelweis University, Budapest, Hungary
7: Institute of Genetics, Cell - and Immunobiology, Semmelweis University, Budapest, Hungary
8: Department of Plant Physiology and Molecular Plant Biology, ELTE Eötvös Loránd University, Budapest, Hungary
9: Biological Nanochemistry Research Group, Institute of Materials and Environmental Chemistry, HUN-REN Research Centre for Natural Sciences, Budapest, Hungary
10: Department of Physical Chemistry and Materials Science, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Budapest, Hungary
11: Department of Biophysics and Radiation Biology - HUN-REN Office for Supported Research Groups, Faculty of Medicine, Semmelweis University, Budapest, Hungary
12: HUN- REN-SU Translational Extracellular Vesicle Research Group, Budapest, Hungary

Bemutatás módja

Poszter

Szekció

Poster Session 2.B - Molecular Medicine

Language of the presentation

Hungarian

Preferred session

Molecular Medicine

Összefoglaló szövege

Antifibrotic Effects of Spirulina maxima Derived Extracellular Vesicles

Abstract

Introduction

Spirulina maxima (Sm) is a nutrient-rich blue-green microalga known for its well-established antioxidant and anti-inflammatory properties. Recent studies suggest that extracellular vesicles (EVs) derived from algae may play a role in mediating these effects, however their specific involvement in fibrosis remains unclear.

Aims

The present study aimed to characterize small EVs produced by Sm (Sm_sEVs) and evaluate their antifibrotic effects in vitro and in vivo.

Methods

Sm_sEVs were isolated from Sm culture medium using tangential flow filtration (TFF) followed by size exclusion chromatography (SEC). Vesicles were characterized by nanoparticle tracking analysis (NTA), transmission electron microscopy, immunogold TEM, surface enhanced IR spectroscopy, and flow cytometry. Cellular uptake of DiI labeled sEVs by human primary peritoneal mesothelial cells (P MCs) and peritoneal fibroblasts (P FBs) was assessed using fluorescence microscopy. Antifibrotic effects were evaluated by immunofluorescence staining, MTT proliferation assays, and Sirius Red collagen accumulation assays. In vivo efficacy was examined in a chlorhexidine digluconate induced mouse model of peritoneal fibrosis.

Results

Sm sEVs were small, spherical vesicles (~150 nm) containing PARK7 and HSP70 proteins. They were efficiently internalized by P MCs and P FBs. Moreover, Sm sEVs inhibited PDGF B–induced proliferation in both cell types, as well as TGF β–induced mesenchymal transition in P MCs and collagen production in P FBs. In vivo, Sm sEV treatment significantly reduced fibrotic changes.

Conclusion

Sm_sEVs exhibit strong antifibrotic activity, highlighting their potential as an algae based therapeutic strategy for fibrotic diseases.

Funding

This research was supported by K-142728 (AJSZ), K-131594 (JM), ADVANCED 150767 (EB), ADVANCED-150077 (ZV), ADVANCED-152698 (JM), ADVANCED- 152112 (TV), TKP2021-EGA-24 (AJSZ), TKP2021-EGA-23 (ÉP), TKP2021-EGA-31 (ZV), STARTING
150629 (ZV), EKÖP-2025-367 (CS); EKÖP-2025-376 (PB); VEKOP-2.3.2-162016-00002 (EB), VEKOP-2.3.3-15-2017-00016 (EB), RRF-2.3.121-2022-00003 (EB), Hungarian Academy of Sciences, János Bolyai Research Scholarship (BS, TV, ZV).

University

Semmelweis University

Supervisor

Dr. Ádám Vannay

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

phd.section.field

in doctoral studies before complex exam (PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

9712

Start

18:18

End

18:21