PhD Scientific Days 2026

Poster Session 3.J - Theoretical and Translational Medicine

Studying Neuronal Autophagy in Human Aging using Induced Neurons Directly Reprogrammed from Adult Human Dermal Fibroblasts

Előadó neve

Mr. Zsoldos, Roland

Neptune code

BLPMAD

Előadó munkahelye

HCEMM-SU Neurobiology and Neurodegenerative Diseases Research Group, Institute of Clinical Pathophysiology Semmelweis University

Előadó telefonszáma

06308478558

Előadó e-mail címe

zsoldos.roland@semmelweis.hu

Az előadás címe

Studying Neuronal Autophagy in Human Aging using Induced Neurons Directly Reprogrammed from Adult Human Dermal Fibroblasts

Szerző(k) neve és munkahelye

Roland Zsoldos¹, Kinga Vörös¹, Zsófia Koltai¹, Chandramouli Muralidharan², Anikó Göblös³, Idris J. Jimoh⁴, Bence Király⁵, Anna Anoir Abbas¹, Balázs Kis¹, Ágnes Varga¹, Csaba Kerepesi⁵, Johan Jakobsson², Mária Judit Molnár⁴, Lajos Kemény³, Karolina Pircs¹

1: HCEMM-SU Neurobiology and Neurodegenerative Diseases Research Group, Institute of Clinical Pathophysiology Semmelweis University
2: Laboratory of Molecular Neurogenetics, Lund University, Lund, Sweden
3: HCEMM-Szeged University, Szeged, Hungary
4: Institute of Genomic Medicine and Rare Disorders, Faculty of Medicine, Semmelweis University, Budapest, Hungary,
5: HUN-REN-SZTAKI-SU Rejuvenation Research Group, Budapest, Hungary

Bemutatás módja

Poszter

Szekció

Poster Session 3.J - Theoretical and Translational Medicine

Language of the presentation

English

Preferred session

Theoretical and Translational Medicine

Összefoglaló szövege

Age is the greatest risk factor for neurodegenerative diseases (NDDs), yet the
molecular links between physiological aging and NDD pathogenesis remain poorly
understood. Autophagy- a lysosomal degradation pathway essential for maintaining cytoplasmic homeostasis- declines with age and contributes to neuronal dysfunction.
To investigate aging alters autophagic flux in human neurons, we utilized direct
reprogramming of human dermal fibroblasts, generating induced neurons (iNs), a method that retains donor-specific genetic and epigenetic signatures. We generated iNs from a cohort of 54 healthy donors ranging in age from 24 to 86 years. Neuronal identity was verified by immunocytochemistry and high-content automated microscopy using neuronal (TAU) and autophagy-specific markers (BECLIN1, LC3, P62, LAMP1) under both basal and stress-induced conditions. Our results indicate donor specific alterations in autophagy. We identified distinct clusters of stress-induced autophagic flux in the case of both young and old donor groups. These variations were observed in autophagosome formation, turnover, as well as in the lysosomal degradation step. These findings suggest different trajectories of age-related decline in neuronal homeostasis, which may contribute to increased vulnerability to age-related neurodegeneration. Currently, we are validating these findings across the full
donor cohort. In parallel, we are conducting multi-omic autophagy profiling using a
range of molecular assays including genome-wide DNA methylation arrays, bulk RNA- sequencing, mass spectrometry, and metabolomics to compare young and old iNs.
Our long-term goal is to identify key regulators of autophagy and explore
rejuvenation strategies. This approach could inform future therapies for NDDs, where impaired autophagy and accelerated aging are often observed.

This work was also supported by the SE 250+ PhD Excellence Grant, SE250-2026-04 by Semmelweis University.

University

Semmelweis University

Supervisor

Dr. Karolina Pircs

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

phd.section.field

in doctoral studies before complex exam (PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

8817

Start

14:00

End

14:03