PhD Scientific Days 2026

Poster Session 2.G - Pharmaceutical Sciences and Health Technologies

Phytochemical Characterization and Investigation of the Biological Activity of Osage orange (Maclura Pomifera L.)

Előadó neve

Dr. Osztie, Rita

Neptune code

IT24UX

Előadó munkahelye

Department of Pharmacognosy

Előadó telefonszáma

+36303541199

Előadó e-mail címe

osztie.rita@stud.semmelweis.hu

Az előadás címe

Phytochemical Characterization and Investigation of the Biological Activity of Osage orange (Maclura Pomifera L.)

Szerző(k) neve és munkahelye

Rita Osztie^1,2, Tamás Czeglédi^1,2, Pegah Poursafar¹, Imre Boldizsár^1,2,3, Márta Kraszni^2,4, Szilvia Bősze^5,6, Ágnes Alberti^1,2

1: Department of Pharmacognosy, Semmelweis University, Knézits utca 14, 1092 Budapest, Hungary
2: Center for Pharmacology and Drug Research & Development, Semmelweis University, 1085 Budapest, Hungary
3: Department of Plant Anatomy, Institute of Biology, Eötvös Loránd University, Pázmány Péter sétány 1/C, 1117 Budapest, Hungary
4: Department of Pharmaceutical Chemistry, Semmelweis University, Hőgyes Endre utca 7-9, 1092 Budapest, Hungary
5: HUN-REN-ELTE Research Group of Peptide Chemistry, Hungarian Research Network, Eötvös Loránd University, Pázmány Péter sétány 1/A, 1117 Budapest, Hungary
6: Department of Genetics, Cell- and Immunobiology, Semmelweis University, Nagyvárad tér 4, 1089 Budapest, Hungary

Bemutatás módja

Poszter

Szekció

Poster Session 2.G - Pharmaceutical Sciences and Health Technologies

Language of the presentation

Hungarian

Preferred session

Pharmaceutical Sciences and Health Technologies

Összefoglaló szövege

Introduction: Plant-derived metabolites continue to be important sources of potential active pharmaceutical ingredients (APIs) today. Following this approach, our work aimed to investigate Osage orange (Maclura pomifera (Raf.) C.K.Schneid), which is used as both a horticultural plant species and a raw material in wood industry. We focused on parts of the plant that are currently not made use of and are treated as waste. Aligned with modern environmental sustainability efforts, their utilization in the pharmaceutical industry could become possible.
Aims: The aim of our study was the polyphenol profiling of Maclura pomifera and the characterization of the membrane permeability, stability, and antiproliferative properties of its main isolated compounds.
Methods: Extracts were prepared from different plant parts using solvents of varying polarity and analyzed by UHPLC-Orbitrap-MS. Compounds were isolated by flash chromatography and semi-preparative RP-HPLC and identified by HR-MS and NMR. Membrane permeability was assessed using the PAMPA-BBB and PAMPA-GI models. Chemical stability of the isolated compounds was evaluated at physiological pH values (pH 7.4, 6.8, and 1.2). The antiproliferative activity was measured by the Alamar Blue assay.
Results: Four major compounds, the prenylated isoflavones osajin, pomiferin, 6,8-diprenylorobol, and 6,8-diprenylgenistein were isolated. The ability of the Osage orange compounds to cross membranes by passive diffusion was investigated for the first time. Results showed no membrane permeation either in the PAMPA-BBB or in the PAMPA-GI model; compounds were undetectable in the acceptor phase and were present only at low concentrations in the donor phase. Subsequent stability studies indicated that most compounds are unstable at the investigated pH values. All compounds were unstable at pH 7.4, and among acidic conditions (pH 1.2). At pH 6.8, pomiferin and 6,8-diprenylorobol showed significant degradation (p<0.05), while osajin and 6,8-diprenylgenistein were stable.
Conclusion: In our work, we provided new data on the chemical and biological characteristics of prenylated isoflavonoids from M. pomifera, which may lay the foundation for the further investigation of the plant as the source of drug candidate compounds.

University

Semmelweis University

Supervisor

Dr. Alberti-Dér Ágnes

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

phd.section.field

in doctoral studies after complex exam (PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

9007

Start

18:30

End

18:33