PhD Scientific Days 2026

Poster Session 2.M - Neurosciences

Transient protein expression in the brain via lipid nanoparticle-mediated mRNA delivery

Előadó neve

Ms. Somogyi, Fanni, MSc

Neptune code

E58ZQ3

Előadó munkahelye

HUN-REN Research Centre for Natural Sciences

Előadó telefonszáma

06702686813

Előadó e-mail címe

vereslfanni@gmail.com

Az előadás címe

Transient protein expression in the brain via lipid nanoparticle-mediated mRNA delivery

Szerző(k) neve és munkahelye

Fanni Somogyi^1,2, Virág György², Hella Czigány^2,3, Dávid Jólesz^2,3, Dániel Hillier^2,3

1: János Szentágothai Doctoral School of Neurosciences, Semmelweis University, Budapest, Hungary
2: HUN-REN Research Centre for Natural Sciences, Budapest, Hungary
3: Faculty of Information Technology and Bionics, Pázmány Péter Catholic University, Budapest, Hungary

Bemutatás módja

Poszter

Szekció

Poster Session 2.M - Neurosciences

Language of the presentation

English

Preferred session

Neurosciences

Összefoglaló szövege

Transient protein expression in the brain is desirable when prolonged activity is unnecessary, biologically unfavorable, or experimentally limiting. This is particularly relevant for applications that require short-term protein function and for paradigms in which repeated interventions within the same brain region are advantageous. In this context, mRNA delivery offers a potentially flexible alternative to viral approaches by enabling controlled, non-permanent expression.

Here, we investigate lipid nanoparticle (LNP)-mediated mRNA delivery as a platform for transient protein expression in the brain. We assess selected LNP formulations with respect to delivery performance, expression kinetics, and local tissue response. To characterize these effects, we combine longitudinal in vivo imaging with post hoc histological analysis, allowing us to evaluate protein expression over time alongside markers of immune activation and cellular integrity.

Our findings show that LNP-mediated mRNA delivery can achieve robust but time-limited protein expression in the brain. We further observe formulation-dependent differences in expression efficiency and host response, indicating that nanoparticle composition is a critical determinant of performance in neural tissue. Together, these results support LNP-based mRNA delivery as a promising strategy for transient, localized protein expression in the brain and provide a basis for the further optimization of non-viral gene delivery approaches in neuroscience.

Supported by the following grants: 2019-2.1.7-ERA-NET-2021-00047, the Lendület (“Momentum") Programme of the Hungarian Academy of Sciences, Excellence 151368 from Ministry of Innovation and Technology of Hungary (NRDI fund), CELSA/24/020 and KSZF-161/2024 to DaH. The scientific research and results published here were reached with the sponsorship of Gedeon Richter Talentum Foundation in the framework of Gedeon Richter Excellence PhD Scholarship of Gedeon Richter to FS.

University

Semmelweis University

Supervisor

Dániel Hillier

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

phd.section.field

in doctoral studies after complex exam (PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

8099

Start

18:48

End

18:51