PhD Scientific Days 2026

Poster Session 2.G - Pharmaceutical Sciences and Health Technologies

PVA Nanofibers as a Carrier Matrix for Plant-Derived Extracellular Vesicles

Előadó neve

Dr. Kürtösi, Balázs

Neptune code

EHTF7Q

Előadó munkahelye

University Pharmacy Department of Pharmacy Administration, Semmelweis University

Előadó telefonszáma

06302889332

Előadó e-mail címe

kurtosi.balazs@phd.semmelweis.hu

Az előadás címe

PVA Nanofibers as a Carrier Matrix for Plant-Derived Extracellular Vesicles

Szerző(k) neve és munkahelye

Dr. Kürtösi Balázs¹, Dr. Alpár Alán², Dr. Buzás Edit³, Fekete Nóra³, Dela Khamari³, Dr. Kállai Nikolett⁴, Csukás-Berta Nicolett⁴, Dr. Virág Dávid⁵

1: University Pharmacy Department of Pharmacy Administration, Semmelweis University
2: Department of Anatomy, Histology and Embryology Semmelweis University
3: Institute of Genetics, Cell- and Immunobiology
4: Department of Pharmaceutics Semmelweis University, Faculty of Pharmacy
5: Hungarian Research Network

Bemutatás módja

Poszter

Szekció

Poster Session 2.G - Pharmaceutical Sciences and Health Technologies

Language of the presentation

Hungarian

Preferred session

Pharmaceutical Sciences and Health Technologies

Összefoglaló szövege

Introduction: Extracellular vesicles (EVs) are lipid bilayer-enclosed structures produced by all cells. EVs play an important role in the transport of molecules such as proteins, lipids, and nucleic acids during intercellular communication. This characteristic makes them promising candidates for drug delivery applications, owing to their scalable and cost-effective production and low immunogenicity.
Aims: The aim of this work was to develop a novel drug delivery system that combines plant-derived extracellular vesicles with a polymer-based nanofibrous matrix.
Methods: Commercially sourced Aloe vera-derived extracellular vesicles were characterized using Nanoparticle Tracking Analysis (NTA), flow cytometry (including Annexin V labelling, membrane-integrity assessment via Triton X-100 treatment). Polyvinyl alcohol (PVA, Mw ~130,000 g/mol) nanofibers were prepared by electrospinning at a polymer concentration of 12 w/w%, with process parameters optimized to reproducible fiber morphology. EVs were stained with lipophilic fluorescent dye Vybrant™ DiD and incorporated into the PVA fiber matrix. Fiber morphology was analyzed by scanning electron microscopy (SEM), while vesicle distribution within the nanofibers was visualized by confocal laser microscopy.
Results: NTA measurements confirmed a monodisperse vesicle distribution with an average hydrodynamic diameter of 157 nm (range 100–250 nm) and a concentration of 3.1 × 10⁹ particles/mL. Nanofibers produced from the 12% PVA solution were bead-free and homogeneous, with a mean diameter of 300 nm. Confocal microscopy verified the successful incorporation of lipid-stained extracellular vesicles into the polymer matrix. Flow cytometry measurements confirmed that the Aloe vesicles were annexin V positive, suggesting a promising approach for vesicle labeling.
Conclusion :Plant-derived extracellular vesicles were successfully characterized and incorporated into PVA nanofibers, yielding a novel bio-nanocarrier system. The findings underscore the need for complementary analytical methods and the identification of plant-specific surface markers to achieve more specific EV characterization. Future work will focus on optimizing vesicle drug loading with various small molecules and performing dissolution and stability studies.

University

Semmelweis University

Other university, not listed above

-

Supervisor

Dr. Zelkó Romána; Dr Nochta-Kazsoki Adrienn Katalin

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

phd.section.field

in doctoral studies after complex exam (PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

9809

Start

18:42

End

18:45