PhD Scientific Days 2026

Poster Session 2.E - Pathological and Oncological Sciences

Development of 3D Bioprinted Melanoma Models

Előadó neve

Gábriel, Zsófia Helga, MSc

Neptune code

IFP7HM

Előadó munkahelye

Department of Pathology and Experimental Cancer Research

Előadó telefonszáma

+36308207650

Előadó e-mail címe

gabriel.zsofia@phd.semmelweis.hu

Az előadás címe

Development of 3D Bioprinted Melanoma Models

Szerző(k) neve és munkahelye

Zsófia Gábriel¹, Viktória Varga¹, Dorottya Moldvai¹, Anna Sebestyén¹

1: Department of Pathology and Experimental Cancer Research

Bemutatás módja

Poszter

Szekció

Poster Session 2.E - Pathological and Oncological Sciences

Language of the presentation

English

Preferred session

Pathological and Oncological Sciences

Összefoglaló szövege

Introduction:

The high metastatic potential and chemoresistance of melanoma warrant new experimental models, as traditional 2D/3D cultures and xenografts fail to accurately recapitulate the human tumor microenvironment and immune signaling. These underscore the importance of 3D bioprinted model developments as physiologically more relevant platforms for melanoma research.

Aims:

The main goal of the research is to establish long-term 3D bioprinted melanoma cultures for drug response analysis and further complex model development. The initial steps of the study focused on the selection of the bioink for further printing process since it has a great impact on cell viability and the stability of the printed scaffold.

Methods:

Three different biogels were tested (1% alginate + 3% gelatin, 3% alginate + 1% gelatin, and a collagen-based ink) on the A2058 human melanoma cell line to explore their compatibility with the cells. For bioink selection the tumor cells [10M/ml] were mixed with the biomaterials, and drops of the mixture were placed in a 96-well plate. Cell growth was monitored for 3 weeks and documented on every 3rd-4th day (day 3, 6, 9, 13, 16 and 20) applying a phase contrast microscope.

Results:

The cells showed a steady growth in the bioinks over the 3-week period. Over this time, some cells crawled out of the alginate-based inks, indicating that A2058 melanoma cells prefer collagen as a scaffold matrix, which supports better cell retention. Spheroids and tissue-like structures formed within the gels, making this model appropriate for further 3D bioprinting.

Conclusions:

Our findings demonstrate that the bioink composition affects cell behaviour. Collagen-based matrices provide a better environment for maintaining cell viability and structural integrity. This platform holds potential for improved drug testing and a more accurate investigation of melanoma tumor biology.

Funding: NKFIH-142799 project; 2025-2.1.1-EKÖP-2025-00014; SE250_2026-129

University

Semmelweis University

Supervisor

Anna Sebestyén, DSc

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

phd.section.field

in doctoral studies before complex exam (PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

9730

Start

18:42

End

18:45