PhD Scientific Days 2026

Poster Session 3.N - Neurosciences

Survival of human grafted neuronal progenitor cells in human cortical organotypic slice cultures

Előadó neve

Ms. Stelcz, Rebeka, MSc

Neptune code

SA70CG

Előadó munkahelye

HUN-REN Research Centre for Natural Sciences

Előadó telefonszáma

+36 1 382 6807

Előadó e-mail címe

stelcz.rebeka@phd.semmelweis.hu

Az előadás címe

Survival of human grafted neuronal progenitor cells in human cortical organotypic slice cultures

Szerző(k) neve és munkahelye

Rebeka Stelcz^1,2, Vera Deli², Katalin Tóth^1,2, Eszter Juhász², Orsolya Farkas², Hanga Dormán², Attila Bagó³, Loránd Erőss³, Boglárka Hajnal³, István Ulbert^2,3,4, Ágota Apáti², Lucia Wittner^2,3

1: Semmelweis University, János Szentágothai Neurosciences Division
2: HUN-REN Research Centre for Natural Sciences
3: Semmelweis University Clinics of Neurosurgery and Neurointervention
4: Péter Pázmány Catholic University Faculty of Information Technology and Bionics

Bemutatás módja

Poszter

Szekció

Poster Session 3.N - Neurosciences

Language of the presentation

English

Preferred session

Neurosciences

Összefoglaló szövege

Introduction: Organotypic slice cultures derived from postoperative human brain tissue offer a unique long-term model for studying neuronal development and integration under near-physiological conditions. This system supports tissue viability for several weeks, enabling investigations of optogenetic tools and stem cell integration.
Aims: In this study, GFP-expressing human induced pluripotent stem cell (iPSC) derived neural progenitor cells (NPCs) were injected into human cortical organotypic slices.
Methods: The survival, differentiation, and integration of the NPCs into the existing neuronal network were analysed using confocal microscopy. To enhance integration, we optimised the culturing medium composition, method, and timing of the injection.
Results: In most cases, the NPCs initiated neuronal polarisation by growing neuronal protrusions; however, round cells with glial morphology were also observed. NPCs survived mainly at the tissue margins and at the injection site, while some migrated deeper into the tissue. These findings demonstrate that NPCs can survive and develop neuronal phenotypes in human cortical slice cultures for up to five weeks without the need for additional trophic factors.
Conclusion: Based on our preliminary findings, our system appears to be a promising platform for studying cell integration and circuit formation in stem cell-based therapies.

Funding: NKFIH K137886, Advanced-150799, SOTE250+, Gedeon Richter Excellence PhD Scholarship

University

Semmelweis University

Supervisor

Lucia Wittner

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

phd.section.field

in doctoral studies before complex exam (PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

9141

Start

14:00

End

14:03