PhD Scientific Days 2026

Poster Session 3.H - Pharmaceutical Sciences and Health Technologies

Novel Engineered Fusogenic Liposome-Based Antioxidant Delivery Improves Blood–Brain Barrier Integrity in Aging

Előadó neve

Dr. Csík, Boglárka

Neptune code

CNNG6W

Előadó munkahelye

Faculty of Medicine, Semmelweis University, Institute of Preventive Medicine and Public Health, Budapest, Hungary

Előadó telefonszáma

+36304930480

Előadó e-mail címe

csik.boglarka@semmelweis.hu

Az előadás címe

Novel Engineered Fusogenic Liposome-Based Antioxidant Delivery Improves Blood–Brain Barrier Integrity in Aging

Szerző(k) neve és munkahelye

Dr. Csík Boglárka^1,2,3, Shanmugarama Santny^2,3, Gronemann Till⁴, Dr. Patai Roland^2,3, Dr. Nyúl-Tóth Ádám^2,3, Dr. Ungvári Zoltán^2,3,5

1: Semmelweis University, Health Sciences Program, Doctoral College, Budapest, Hungary
2: University Of Oklahoma Health Sciences Center, Department of Neurosurgery, Vascular Cognitive Impairment, Neurodegeneration and Healthy Brain Aging Program, Oklahoma City, United States
3: University Of Oklahoma Health Sciences Center, Oklahoma Center for Geroscience and Healthy Brain Aging, Oklahoma City, United States
4: Forschungszentrum Jülich GmbH, Institute of Biological Information Processing: Mechanobiology (IBI-2), Jülich, Germany
5: Faculty of Medicine, Semmelweis University, Institute of Preventive Medicine and Public Health, Budapest, Hungary

Bemutatás módja

Poszter

Szekció

Poster Session 3.H - Pharmaceutical Sciences and Health Technologies

Language of the presentation

English

Preferred session

Pharmaceutical Sciences and Health Technologies

Összefoglaló szövege

Introduction: Vascular defects contribute to age-related neurodegeneration and cognitive impairment. Blood–brain barrier (BBB) breakdown and neurovascular uncoupling lead to cerebral blood flow deficits, neuroinflammation, and decreased cognitive function. The naturally occurring polyphenol resveratrol (RSV) attenuates oxidative stress, thus improving vascular health. However, its oral bioavailability is low; therefore, it requires an alternative delivery system to enhance the efficiency of antioxidant and anti-inflammatory polyphenols.
Aims: We introduced a novel fusogenic liposomal (FL) system with an engineered apolipoprotein E (ApoE)-based protein corona to enhance RSV bioavailability and directly target cerebrovascular endothelial cells, thereby increasing RSV uptake and improving delivery to protect the cerebral microvasculature during aging. Our central hypothesis was that endothelial barrier dysfunction can be reduced by ApoE-FLs loaded with RSV, thus mitigating vascular cognitive dysfunction.
Methods: Biodistribution of fluorescently labeled FL and ApoE-FL was assessed ex vivo in multiple organs following in vivo administration. Organs were collected, processed into histological sections, imaged using confocal microscopy, and analyzed with quantitative image analysis. In vivo multiphoton imaging was used to assess the BBB longitudinally, and the radial arm water maze was used to assess cognition.
Results: ApoE-FL-RSV showed significantly greater accumulation in brain endothelial cells in vivo compared to non-targeted FLs. In aged mice, both FL and ApoE-FL treatment improved BBB integrity and cognition.
Conclusion: Targeted delivery of RSV via ApoE-FL improves cerebrovascular function and mitigates cognitive decline in aging, representing a promising therapeutic strategy against age-related vascular impairment and cognitive decline.
Funding: EKÖP-2025-703, University Research Scholarship Programme of the Ministry of Culture and Innovation from the source of the National Research, Development and Innovation Fund, American Heart Association (ANT: AHA834339), National Institute on Aging (RF1AG072295, R01AG055395, R01AG068295; R01AG070915, K01AG073614), the National Institute of Neurological Disorders and Stroke (R01NS100782)

University

Semmelweis University

Supervisor

Prof. Dr. Ungvári Zoltán

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

phd.section.field

in doctoral studies after complex exam (PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

8945

Start

14:12

End

14:15