PhD Scientific Days 2026

Theoretical and Translational Medicine 2.

Development of a Modular E. coli BL21(DE3)-Derived OMV Platform for Future Neoantigen Delivery

Előadó neve

Horváth, Mátyás, MSc

Neptune code

EPVJEV

Előadó munkahelye

Translational Medicine Institute, Semmelweis University

Előadó telefonszáma

06307894920

Előadó e-mail címe

matyiest@gmail.com

Az előadás címe

Development of a Modular E. coli BL21(DE3)-Derived OMV Platform for Future Neoantigen Delivery

Szerző(k) neve és munkahelye

Mátyás Horváth¹

1: Translational Medicine Institute, Semmelweis University

Bemutatás módja

Szóbeli

Szekció

Theoretical and Translational Medicine 2.

Language of the presentation

Hungarian

Preferred session

Theoretical and Translational Medicine

Összefoglaló szövege

Introduction: Outer membrane vesicles (OMVs) are promising self-adjuvant nanocarriers for cancer immunotherapy because they can deliver cargo to antigen-presenting cells and support tumour-specific T-cell responses.

Aims: To develop a modular E. coli BL21(DE3)-based OMV platform for future personalised neoantigen delivery by replacing a validated fluorescent model cargo with tumour-specific antigens.

Methods: SpyTag-mScarlet was expressed in the periplasm of E. coli BL21(DE3) as a proof-of-concept cargo. Expression and construct integrity were assessed by SDS-PAGE, fluorescence measurements, colony phenotype, and PCR using T7 primers. Based on this validated system, the next step is to couple SpyCatcher to an outer membrane protein, followed by replacement of mScarlet with selected neoantigens and subsequent mouse studies. Vesiculation and cargo enrichment are currently being optimised. In parallel, we plan to examine osmotic-stress-induced OMP pattern changes and their immunological effects in Caco-2 cells.

Result: Preliminary results demonstrate inducible OMV production with visually detectable mScarlet cargo expression. Protein expression was supported by the presence of magenta colonies, the SDS-PAGE pattern, and fluorescence readouts in both the pellet and the OMV isolate. In addition, colony PCR after transformation yielded the expected amplicon length with T7 primers. These findings indicate successful periplasmic cargo expression and provide a workable basis for further OMV engineering.

Conclusion: Our preliminary data establish the technical feasibility of a BL21(DE3)-derived OMV platform carrying an inducible periplasmic model cargo. Together with published evidence that engineered OMVs can induce antigen-specific cellular immunity and suppress tumour growth in mice, and that BL21(DE3)-derived OMVs can enhance intratumoural CD8+ T-cell infiltration and synergise with anti-PD-1 therapy, this system provides a rational foundation for future neoantigen-loaded OMV vaccines.

University

Semmelweis University

Supervisor

Lohinai Zoltán

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

phd.section.field

in doctoral studies before complex exam (PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

9731

Start

09:30

End

09:40