PhD Scientific Days 2026

Poster Session 2.E - Pathological and Oncological Sciences

Detection of Helicobacter Pylori and Its Clarithromycin Resistance from Histologically Helicobacter Pylori-Negative Gastric Biopsy Samples by Quantitative Real-Time PCR

Előadó neve

Biró, Adrienn

Neptune code

T0PA29

Előadó munkahelye

Department of Pathology, Forensic and Insurance Medicine, Semmelweis University

Előadó telefonszáma

+36304868448

Előadó e-mail címe

biro.adrienn@phd.semmelweis.hu

Az előadás címe

Detection of Helicobacter Pylori and Its Clarithromycin Resistance from Histologically Helicobacter Pylori-Negative Gastric Biopsy Samples by Quantitative Real-Time PCR

Szerző(k) neve és munkahelye

Adrienn Biró¹, Anita Kurucz¹, Ákos Jakab¹, Evelin Barna¹, Ildikó Szirtes¹, Flóra Amina Elamin¹, György Miklós Buzás², Veronika Papp³, Tamás Vass³, Attila Szijártó³, András Kiss¹, Éva Kocsmár¹, Gábor Lotz¹

1: Department of Pathology, Forensic and Insurance Medicine, Semmelweis University, Budapest, Hungary
2: Department of Gastroenterology, Ferencváros Health Centre, Budapest, Hungary
3: Department of Surgery, Transplantation and Gastroenterology, Semmelweis University, Budapest, Hungary

Bemutatás módja

Poszter

Szekció

Poster Session 2.E - Pathological and Oncological Sciences

Language of the presentation

English

Preferred session

Pathological and Oncological Sciences

Összefoglaló szövege

Patients infected with Helicobacter pylori (HP) may present low-density infections that remain undetected by conventional tests, such as histological examination.

Our aim was to perform HP qPCR on formalin-fixed paraffin-embedded (FFPE) gastric tissue samples from patients diagnosed as HP negative by histology. For qPCR-positive cases, we investigated whether there were any symptoms associated with HP and which symptoms might indicate the need for additional qPCR testing.

A total of 480 FFPE gastric biopsy samples—previously reported as HP negative—were collected. DNA was isolated, and qPCR was performed using our FFPE optimized HPJ primer pair targeting the bacterial 23S rRNA gene. For HP positive samples, clinical symptoms, diagnoses, and histopathological findings were reviewed.

Among the 480 histologically HP negative samples, HP infection was detected in 15 cases (3.13%) by HPJ qPCR. Reviewing these patients’ records revealed gastritis in 12 and erosion in 11 cases. Across all histologically HP negative samples, gastritis was diagnosed in 118 cases, of which 10.17% were qPCR positive, while erosion was found in 81 cases, with 13.58% qPCR positive. Of the 15 HP-positive cases, clarithromycin resistance was detected in five (33%); of these, four were homoresistant and one was heteroresistant.

The presence of Helicobacter pylori on the mucosa is not continuous, and low bacterial density may make histological detection impossible in certain cases. Methods based on extracted bacterial DNA offer a solution to this problem. HPJ qPCR successfully identified HP infection in patients with HP associated symptoms whose biopsies were histologically negative. Therefore, HPJ qPCR can serve as a complementary tool in histologically HP-negative cases, particularly when HP-related clinical findings and/or histological changes are present.

The study was funded by the K_22 142604 grant of the National Research, Development and Innovation Office (Hungary), as well as the 2025-2.1.1-EKÖP-2025-00014 University Research Scholarship Programme (grant number EKÖP-2025-401) and KDP-2023 Funding Scheme (2023-2.1.2-KDP-2023-00016) of the Ministry for Culture and Innovation from the source of the National Research, Development and Innovation Fund, Hungary.

University

Semmelweis University

Supervisor

Prof. Dr. Gábor Lotz

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

phd.section.field

in doctoral studies after complex exam (PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

8014

Start

18:18

End

18:21