PhD Scientific Days 2026

Poster Session 3.H - Pharmaceutical Sciences and Health Technologies

miR-450a as a Cardioprotective MicroRNA: Validation and Mechanism of Action

Előadó neve

Szabó, Márta, MSc

Neptune code

I8DJ5G

Előadó munkahelye

Semmelweis University - Department of Pharmacology and Pharmacotherapy

Előadó telefonszáma

06204300271

Előadó e-mail címe

szabo.marta2@semmelweis.hu

Az előadás címe

miR-450a as a Cardioprotective MicroRNA: Validation and Mechanism of Action

Szerző(k) neve és munkahelye

Márta Szabó¹, Boglárka Frankó¹, Regina Nagy¹, Áron Gyovai², András Makkos², Zoltán Bereczki¹, Bence Ágg², Anikó Görbe², Péter Ferdinandy²

1: 1Department of Pharmacology and Pharmacotherapy, Semmelweis University, H-1085 Budapest, Hungary; 2Center for Pharmacology and Drug Research & Development, Semmelweis University, H-1085 Budapest, Hungary
2: 1Department of Pharmacology and Pharmacotherapy, Semmelweis University, H-1085 Budapest, Hungary; 2Center for Pharmacology and Drug Research & Development, Semmelweis University, H-1085 Budapest, Hungary; 3Pharmahungary Group, H-6722 Szeged, Hungary

Bemutatás módja

Poszter

Szekció

Poster Session 3.H - Pharmaceutical Sciences and Health Technologies

Language of the presentation

English

Preferred session

Pharmaceutical Sciences and Health Technologies

Összefoglaló szövege

Introduction: Acute myocardial infarction (AMI) is a leading cause of death worldwide, however, an effective pharmacotherapy for ischemia-reperfusion (I/R) injury remains an unmet medical need. MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression post-transcriptionally and have emerged as promising multi-target therapeutic candidates. Using a translational porcine AMI model, we previously identified cardioprotective miRNAs, termed ProtectomiRs, among which miR-450a was selected for further investigation.
Aims: This study aimed to validate the cardioprotective effects of miR-450a across species and to explore the molecular pathways underlying its action.
Methods: ProtectomiR candidates identified in a clinically relevant porcine AMI model were validated in neonatal rat cardiomyocytes (NRCM) and AC16 human cardiomyocytes exposed to simulated I/R. Cells were treated with miR-450a mimic across a concentration range (0.75-100 nM), and cell viability was assessed. Predicted targets were identified using miRNAtarget™ software, followed by Gene Ontology and KEGG pathway enrichment analyses.
Results: miR-450a showed a concentration-dependent protective effect in both NRCM and AC16 cells under simulated I/R conditions. A concentration of 25 nM significantly improved cell viability, while protective effects were also observed at lower concentrations. Bioinformatic analyses identified SMAD2, DAPK2 and SOD2 among the predicted targets, linking miR-450a to TGF-β signalling, apoptosis and redox regulation. Enrichment analysis further highlighted mTOR and phosphatidylinositol signalling pathways, suggesting effects on key cell survival processes.
Conclusion: miR-450a shows cardioprotective effects in both rat and human cardiomyocytes, through the regulation of cell survival-related signalling pathways. These findings support the further development of miR-450a as a potential therapeutic candidate against I/R injury.
Funding: This work was supported by the SE250+ Excellence PhD Scholarship (Semmelweis University), the National Research, Development and Innovation Office (NKFIH, Hungary; K139237 to A.G.), and projects RRF-2.3.1-21-2022-00003, TKP2021-EGA-23, 2024-1.2.3-HU-RIZONT-2024-00026, and 2020-1.1.5-GYORSÍTÓSÁV-2021-00011, with additional support from the European Union and the Semmelweis Lendület 2024 Program.

University

Semmelweis University

Supervisor

Dr. Anikó Görbe

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

phd.section.field

in doctoral studies after complex exam (PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

9103

Start

14:24

End

14:27